Circ_0001806 Promotes the Proliferation, Migration and Invasion of NSCLC Cells Through miR-1182/NOVA2 Axis

Shenni Yi,1,* Zhan Li,1,* Xiuqin Wang,1,* Tiantian Du,1 Xiuhong Chu2 1Department of Respiratory and Critical Medicine, Yantai Yuhuangding Hospital, Yantai, Shandong Province, 264000, People’s Republic of China; 2Department of Nursing, Yantai YEDA Hospital, Yantai, Shandong Province, 264006, People’s Republic of China*These authors contributed equally to this workCorrespondence: Tiantian DuDepartment of Respiratory and Critical Medicine, Yantai Yuhuangding Hospital, East Yuhuangding Road NO. 20, Yantai, Shandong Province, 264000, People’s Republic of ChinaEmail quebi7192@163.comXiuhong ChuDepartment of Nursing, Yantai YEDA Hospital, Taishan Road No. 11, Yantai Development Area, Yantai, Shandong Province, 264006, People’s Republic of ChinaEmail vkr758157@163.comBackground: Circular RNAs (circRNAs) are non-coding RNAs with a covalently closed loop. circRNAs affect the progression of diverse cancers. Nonetheless, circ_0001806 expression and function in non-small cell lung cancer (NSCLC) are undefined.Methods: qRT-PCR was executed to examine circ_0001806, miR-1182 and NOVA2 mRNA expression levels in NSCLC tissues and cells. CCK-8, EdU, cell scratch test and Transwell assay were conducted to examine the viability, multiplication, migration and invasion of NSCLC cell lines H1650 and HCC827. The binding sites between circ_0001806 and miR-1182, miR-1182 and NOVA2 mRNA were predicted by the circular RNA Interactome and TargetScan databases, and the dual-luciferase reporter gene experiment was employed for verification. Western blot was implemented to examine NOVA2 expression.Results: Circ_0001806 expression in NSCLC tissues and cell lines was substantially augmented, while miR-1182 expression was markedly decreased. Circ_0001806 facilitated the multiplication, migration and invasion of H1650 and HCC827 cells, while miR-1182 exerted the opposite effect. Circ_0001806 indirectly enhanced NOVA2 expression by specifically down-modulating miR-1182.Conclusion: Circ_0001806 augments NOVA2 expression by targeting miR-1182 to enhance the multiplication, migration and invasion of NSCLC cells.Keywords: circ_0001806, miR-1182, NOVA2, NSCLC