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Development of Parvifloron D-Loaded Smart Nanoparticles to Target Pancreatic Cancer

Authors :
Ana Santos-Rebelo
Catarina Garcia
Carla Eleutério
Ana Bastos
Sílvia Castro Coelho
Manuel A. N. Coelho
Jesús Molpeceres
Ana S. Viana
Lia Ascensão
João F. Pinto
Maria M. Gaspar
Patrícia Rijo
Catarina P. Reis
Source :
Pharmaceutics, Vol 10, Iss 4, p 216 (2018)
Publication Year :
2018
Publisher :
MDPI AG, 2018.

Abstract

Pancreatic cancer is the eighth leading cause of cancer death worldwide. For this reason, the development of more effective therapies is a major concern for the scientific community. Accordingly, plants belonging to Plectranthus genus and their isolated compounds, such as Parvifloron D, were found to have cytotoxic and antiproliferative activities. However, Parvifloron D is a very low water-soluble compound. Thus, nanotechnology can be a promising delivery system to enhance drug solubility and targeted delivery. The extraction of Parvifloron D from P. ecklonii was optimized through an acetone ultrasound-assisted method and isolated by Flash-Dry Column Chromatography. Then, its antiproliferative effect was selectivity evaluated against different cell lines (IC50 of 0.15 ± 0.05 μM, 11.9 ± 0.7 μM, 21.6 ± 0.5, 34.3 ± 4.1 μM, 35.1 ± 2.2 μM and 32.1 ± 4.3 μM for BxPC3, PANC-1, Ins1-E, MCF-7, HaCat and Caco-2, respectively). To obtain an optimized stable Parvifloron D pharmaceutical dosage form, albumin nanoparticles were produced through a desolvation method (yield of encapsulation of 91.2%) and characterized in terms of size (165 nm; PI 0.11), zeta potential (−7.88 mV) and morphology. In conclusion, Parvifloron D can be efficiently obtained from P. ecklonii and it has shown selective cytotoxicity to pancreatic cell lines. Parvifloron D nanoencapsulation can be considered as a possible efficient alternative approach in the treatment of pancreatic cancer.

Details

Language :
English
ISSN :
19994923
Volume :
10
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Pharmaceutics
Publication Type :
Academic Journal
Accession number :
edsdoj.0157646afad04a0194a41db6436ded37
Document Type :
article
Full Text :
https://doi.org/10.3390/pharmaceutics10040216