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TSPO PET upregulation predicts epileptic phenotype at disease onset independently from chronic TSPO expression in a rat model of temporal lobe epilepsy

Authors :
Daniele Bertoglio
Halima Amhaoul
Joery Goossens
Idrish Ali
Elisabeth Jonckers
Tom Bijnens
Matteo Siano
Leonie wyffels
Jeroen Verhaeghe
Annemie Van der Linden
Steven Staelens
Stefanie Dedeurwaerdere
Source :
NeuroImage: Clinical, Vol 31, Iss , Pp 102701- (2021)
Publication Year :
2021
Publisher :
Elsevier, 2021.

Abstract

Neuroinflammation is a key component of epileptogenesis, the process leading to acquired epilepsy. In recent years, with the development of non-invasive in vivo positron emission tomography (PET) imaging of translocator protein 18 kDa (TSPO), a marker of neuroinflammation, it has become possible to perform longitudinal studies to characterize neuroinflammation at different disease stages in animal models of epileptogenesis. This study aimed to utilize the prognostic capability of TSPO PET imaging at disease onset (2 weeks post-SE) to categorize epileptic rats with distinct seizure burden based on TSPO levels at disease onset and investigate their association to TSPO expression at the chronic epilepsy stage. Controls (n = 14) and kainic acid-induced status epilepticus (KASE) rats (n = 41) were scanned non-invasively with [18F]PBR111 PET imaging measuring TSPO expression. Animals were monitored using video-electroencephalography (vEEG) up to chronic disease (12 weeks post-SE), at which TSPO levels ([3H]PK11195) as well as other post-mortem abnormalities (namely synaptic density ([3H]UCB-J), neuronal loss (NeuN), and neurodegeneration (FjC)) were investigated. By applying multivariate analysis, TSPO PET imaging at disease onset identified three KASE groups with significantly different spontaneous recurrent seizures (SRS) burden (defined as rare SRS, sporadic SRS, and frequent SRS) (p = 0.003). Interestingly, TSPO levels were significantly different when comparing the three KASE groups (p

Details

Language :
English
ISSN :
22131582
Volume :
31
Issue :
102701-
Database :
Directory of Open Access Journals
Journal :
NeuroImage: Clinical
Publication Type :
Academic Journal
Accession number :
edsdoj.00c894885164fe5bc81f163bc88c743
Document Type :
article
Full Text :
https://doi.org/10.1016/j.nicl.2021.102701