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Quantitative analysis of reptation of partially extended DNA in sub-30 nm nanoslits
- Publication Year :
- 2015
-
Abstract
- We observed reptation of single DNA molecules in fused silica nanoslits of sub-30 nm height. The reptation behavior and the effect of confinement are quantitatively characterized using orientation correlation and transverse fluctuation analysis. We show tube-like polymer motion arises for a tense polymer under strong quasi-2D confinement and interaction with surface- passivating polyvinylpyrrolidone (PVP) molecules in nanoslits, while etching- induced device surface roughness, chip bonding materials and DNA-intercalated dye-surface interaction, play minor roles. These findings have strong implications for the effect of surface modification in nanofluidic systems with potential applications for single molecule DNA analysis.<br />Comment: 22 pages. 4 figures
- Subjects :
- Physics - Biological Physics
Condensed Matter - Soft Condensed Matter
Subjects
Details
- Database :
- arXiv
- Publication Type :
- Report
- Accession number :
- edsarx.1502.05115
- Document Type :
- Working Paper