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Cystathionine synthase T833C/844ins68 Polymorphism: A family-based study on down syndromes children

Authors :
TURKYILMAZ, Aysegul
SIMSEK, Selda
ORAL, Diclehan
TEKEŞ, Selahattin
ISI, Hilmi
Source :
Volume: 2, Issue: 2 54-56, International Archives of Medical Research
Publication Year :
2011
Publisher :
Veysi AKPOLAT, 2011.

Abstract

Cystathionine β-synthase (CBS) mediates conversion of homocysteine to cystathionine and deficiency in enzyme activity may be lead to hyperhomocysteinemia/homocystinuria, which are often associated with Down Syndrome (DS). A large number of polymorphisms have been reported in the CBS gene, some of which impair its activity and among these, a T833C polymorphism in cis with a 68 bp insertion at 844 in the exon 8 is found to be associated with mild hyperhomocysteinemia in different ethnic groups. Our aim in the present study is to investigate the association between T833C/844ins68 polymorphism and DS. Methods: Fifty-seven DS cases parents (mothers) were recruited after psychometric evaluation. Peripheral blood was collected after obtaining informed written consent. The T833C/844ins68 polymorphism was investigated by PCR amplification of genomic DNA Results: After PCR Analysis 15 samples were found to have +/- genotype while 42 samples were found to have -/- genotype for CBS 844ins68 polymorphisms. Conclusion: This is the first molecular genetic study of CBS gene dealing with T833C/844ins68 double mutation in DS subjects in our region. The next step is to extended number of cases and to use more controls for T833C/844INS68 polymorphism.

Details

ISSN :
21466033
Database :
OpenAIRE
Journal :
Volume: 2, Issue: 2 54-56, International Archives of Medical Research
Accession number :
edsair.tubitakulakb..f1b741908a1758529f1de4e6bd963e7e