Servikal Sürüntü Örneklerinden ID32 C Kiti İle Saptanmış Concida Albicans Türlerini Tanımlamada PCR Yönteminin Etkinliği

Efficacy of PCR Assay in Diagnosis of Candida Albicans Strains Typed by ID32 C in Servical Smear PreperationIn this study, we aimed to evaluate two different assays used for the confirmation of Candida albicans infections and determine the efficacy of PCR assay in Candida albicans diagnosed by ID32 C kit in servical smear preperation of the patients followed in Gynecology and Obstetric out-patient clinic. Seventy patients (mean age 35) were enrolled in this study. While 10% of the patients have no complaint, chronic flow in 9%of the patients, recurrent vulvovaginal candidosis in 45% of the patients and acute infection in 36% of the patients were found. Samples collected with swab were incubated in SDA at 37°C for 24-48 hours. Yeast colonisations were identified by ID32 C kit and confirmed by a PCR assay using primers sets specific to Candida albicans. Yeast colonisations were observed in 31.4% of 70 patients. Identification of yeast colonisation by ID32 C kit showed C.albicans in 20%, C. krusei in 5.7%, C.glabrata in 4.3% and C. insconspicua in 1.4% of all patients. The PCR test results were found to be positive in 17.5% of the patients. In our study, we found that sensivity of ID32 C kit was better than PCR assay, although this difference was not statisticaly significant.As a result we found that efficacy of PCR assay was proximate to ID32 C kit although this difference was not statisticaly significant.Çalışmamızın amacı üriner tüberküloz tanısı konan hastalarda intravenöz ürografide görülen değişiklikleri ve bunun tanıdaki önemini incelemektir.Üriner tüberküloz tanısı konulan 24 hastada (10 erkek, 14 kadın) intravenöz ürografi tetkiki yapıldı ve değişiklikler incelendi.Üriner tüberkülozlu 24 hastanın 6'smda tetkik sonucu normaldi. İki hastada kalikslerde güve yeniği görünümü. 2 hastada üreterde düzensizlik, bir hastada böbrekte fonksiyon gecikmesi, bir hastada otonefrektomi izlenirken, diğer hastalarda çeşitli kaliksiyel patolojiler saptandı.Normal intravenöz ürografi incelemesi üriner tüberkülozu ekarte ettirmez. Üriner tüberküloz düşünülen hastalarda intravenöz ürografi ile kesin tanı konulamaz. Üriner sistemde olabilecek değişiklikleri incelemek için intravenöz ürografi'den yararlanılır.