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First Report and Comparative Genomics Analysis of a blaOXA-244–Harbouring Escherichia coli Isolate Recovered in American Continent
- Authors :
- Abril, Deisy J.
Moya, Ingrid Gisell Bustos
Marquez-Ortiz, Ricaurte Alejandro
Montero, Diego Fernando Josa
Rozo, Zayda Lorena Corredor
Molina, Isabel Torres
Gómez, Natasha Vanegas
Escobar-Perez, Javier - Publication Year :
- 2019
- Publisher :
- Preprints, 2019.
-
Abstract
- The carbapenemase OXA-244 is a derivate of OXA-48, and its detection is very difficult in laboratories. Here we report the identification and genomic analysis of an Escherichia coli isolate (28Eco12) harbouring the blaOXA-244 gene identified in Colombia, South America. The 28Eco12 isolate was identified during a retrospective study and it was recovered from a patient treated in Colombia. The complete nucleotide sequence was established using the PacBio platform. A comparative genomics analysis with other blaOXA-244–harbouring Escherichia coli strains was performed. The 28Eco12 isolate belonged to sequence type (ST) 38 and its genome was composed of two molecules, a chromosome of 5,343,367 bp and a plasmid of 92,027 bp, which belonged to the incompatibility group IncY and did not harbour resistance genes. The blaOXA-244 gene was chromosomally-encoded and mobilized by an ISR1-related Tn6237 composite transposon. Notably, this transposon was inserted and located within a new genomic island. For our knowledge this is the first report of a blaOXA-244–harbouring Escherichia coli isolate in American continent.Our results suggest that the introduction of the OXA-244-producing E. coli isolate was through clonal expansion of the ST38 pandemic clone. Other isolates producing OXA-244 could be circulating silently on the American continent.
- Subjects :
- medicine_pharmacology_other
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.sharePrprorg..a52a9a969953df5cadb074227e6ce5f0