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Partial characterization of angiotensin II-like material extracted from the rat brain
- Source :
- Journal of Hypertension-Supplement, Journal of Hypertension-Supplement-, Current Science Ltd, 1986, 4 (6), pp.S446-9, Journal of Hypertension-Supplement-, 1986, 4 (6), pp.S446-9
- Publication Year :
- 1986
- Publisher :
- HAL CCSD, 1986.
-
Abstract
- International audience; Angiotensin II (ANG II)-like material was detected in acid extracts of rat brain using radio-immunoassay (RIA) and a radioreceptor assay (RRA). This material, expressed as ANG II equivalents, corresponded to 131 +/- 20 fmol/g and 33 +/- 4 pmol/g as assessed by RIA and RRA respectively. Such quantitative differences indicated that the brain material did not behave as authentic ANG II in both assays, and further chromatographic investigations confirmed this inference. In particular, gel filtration through Sephadex G-25 and TSK Spherogel 3000 SW revealed that the apparent molecular weight of ANG II-like material was much higher (approximately 5000-7000) than that of authentic ANG II. Furthermore, in contrast to the marked hypertension due to ANG II, a decrease in blood pressure (BP) was observed in rats following the systemic administration of chromatographic eluates enriched with brain ANG II-like material.
- Subjects :
- Male
Chemical Phenomena
MESH: Rats
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Radioimmunoassay
Blood Pressure
MESH: Radioimmunoassay
MESH: Brain
Animals
MESH: Animals
MESH: Chemistry, Physical
MESH: Brain Chemistry
Brain Chemistry
MESH: Tissue Extracts
[SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior
Chemistry, Physical
Tissue Extracts
Angiotensin II
Brain
[SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences
Rats, Inbred Strains
MESH: Chemical Phenomena
MESH: Chromatography, Gel
MESH: Blood Pressure
MESH: Rats, Inbred Strains
MESH: Male
Rats
Chromatography, Gel
MESH: Angiotensin II
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- Language :
- English
- ISSN :
- 09521178
- Database :
- OpenAIRE
- Journal :
- Journal of Hypertension-Supplement, Journal of Hypertension-Supplement-, Current Science Ltd, 1986, 4 (6), pp.S446-9, Journal of Hypertension-Supplement-, 1986, 4 (6), pp.S446-9
- Accession number :
- edsair.pmid.dedup....ffec6936a04ecbb449879e51418cb70d