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Time-dependent expression and processing of a hypothetical protein of possible importance for regulation of the Chlamydia pneumoniae developmental cycle

Authors :
Brian Berg Vandahl
Kris Gevaert
Hans Demol
Bart Hoorelbeke
Arne Holm
Jöel Vandekerckhove
Gunna Christiansen
Svend Birkelund
Source :
Aalborg University
Publication Year :
2001

Abstract

Chlamydia pneumoniae is an obligate intracellular human pathogen infecting epithelial cells of the upper respiratory tract. It is a Gram-negative bacteria and has a unique biphasic developmental cycle. In this study, we use two-dimensional gel electrophoresis in combination with radioactive labeling to investigate time-dependent expression and processing of C. pneumoniae proteins. We report on (i) the identification of a hypothetical protein which is expressed late in the developmental cycle and subsequently processed; we speculate that this protein may be of importance for the developmental cycle of Chlamydia; (ii) the identification of the major outer membrane protein in three different variants, which may all be present in vivo.

Details

ISSN :
01730835
Volume :
22
Issue :
9
Database :
OpenAIRE
Journal :
Electrophoresis
Accession number :
edsair.pmid.dedup....f03e11f9b126b71b72a857f25d5efa2b