Aberrant Expression of miR-592 Is Associated with Prognosis and Progression of Renal Cell Carcinoma

Xianbao Lv,1,* Jingang Shen,1,* Zhen Guo,2,* Lingwei Kong,1 Guangchun Zhou,1 Hao Ning3 1Department of Urology, Chengwu People’s Hospital, Heze, Shandong, 274200, People’s Republic of China; 2Department of Urology, Central Hospital Affiliated Shandong First Medical University, Jinan, Shandong, 250033, People’s Republic of China; 3Department of Urology, Shandong Provincial Hospital, Jinan, Shandong, 250021, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hao NingDepartment of Urology Surgery, Shandong Provincial Hospital, No. 324, Jingwu Road, Jinan, Shandong 250021, People’s Republic of ChinaTel +86 531 68776310Email haotiecg4792@163.comPurpose: MicroRNAs have recently reported playing a vital role in the development of cancers. However, the role of miR-592 in renal cell carcinoma (RCC) has not been explored. In this study, the potential role of miR-592 was investigated in RCC.Patients and methods: The expression of miR-592 was evaluated in RCC tissues and cell lines using qRT-PCR assays. The Kaplan-Meier analysis and Cox proportional hazards model analysis was used to analyze the prognostic value of miR-592 in RCC. The effects of miR-592 on cell proliferation, migration, and invasion were determined by cell counting kit-8 (CCK-8) and Transwell assays in vitro.Results: The results showed that miR-592 was significantly increased both in RCC tissues and cell lines. Overexpression of miR-592 was significantly associated with lymph node metastasis, TNM stage, and poor overall survival. And functional studies in two RCC cell lines (786-O and Caki-1) have shown that overexpression of miR-592 promoted cell proliferation, migration, and invasion, while silence of miR-592 inhibited cell proliferation, migration, and invasion. SPRY2 was a direct target of miR-592.Conclusion: Overall, overexpression of miR-592 may be a prognostic biomarker and therapeutic strategy for patients with RCC, which is correlated with the progression of RCC.Keywords: miR-592, renal cell carcinoma, prognosis, proliferation, migration, invasion