Back to Search
Start Over
The invasive pathogen Yersinia pestis disrupts host blood vasculature to spread and provoke hemorrhages
- Source :
- PLoS Neglected Tropical Diseases, Vol 15, Iss 10, p e0009832 (2021), PLoS Neglected Tropical Diseases, PLoS Neglected Tropical Diseases, Public Library of Science, 2021, 15 (10), pp.e0009832. ⟨10.1371/journal.pntd.0009832⟩, PLoS Neglected Tropical Diseases, 2021, 15 (10), pp.e0009832. ⟨10.1371/journal.pntd.0009832⟩
- Publication Year :
- 2021
- Publisher :
- Public Library of Science (PLoS), 2021.
-
Abstract
- Yersinia pestis is a powerful pathogen with a rare invasive capacity. After a flea bite, the plague bacillus can reach the bloodstream in a matter of days giving way to invade the whole organism reaching all organs and provoking disseminated hemorrhages. However, the mechanisms used by this bacterium to cross and disrupt the endothelial vascular barrier remain poorly understood. In this study, an innovative model of in vivo infection was used to focus on the interaction between Y. pestis and its host vascular system. In the draining lymph nodes and in secondary organs, bacteria provoked the porosity and disruption of blood vessels. An in vitro model of endothelial barrier showed a role in this phenotype for the pYV/pCD1 plasmid that carries a Type Three Secretion System. This work supports that the pYV/pCD1 plasmid is responsible for the powerful tissue invasiveness capacity of the plague bacillus and the hemorrhagic features of plague.<br />Author summary The plague bacillus, Yersinia pestis, is a powerful pathogen with a rare invasive capacity and is among the few bacteria capable to provoke disseminated hemorrhages. However, the mechanisms used by this bacterium to cross and disrupt the endothelial vascular barrier remain poorly understood. Recent technical progress in microscopy, associated with the use of original fluorescent mutant in mice, allowed us to develop an innovative model of infection in vivo. This model permitted to look directly into the interaction between Y. pestis and its host vascular system, in 3D reconstructed tissues without physical alteration. We were able to observe the degradation of blood vessels in the draining lymph nodes and to visualize the spreading of the bacteria into secondary organs directly through the vascular barrier. Classical in vitro experiments validated the in vivo observation and demonstrated the role of some of the bacterial components in this phenotype. This work shows an unprecedented visualization of the pathogenesis of Y. pestis and decipher part of the powerful invasiveness capacity of the plague bacillus and the hemorrhagic features of plague.
- Subjects :
- Bacterial Diseases
Confocal Microscopy
Physiology
RC955-962
Pathology and Laboratory Medicine
Vascular Medicine
Mice
Medical Conditions
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Arctic medicine. Tropical medicine
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
Medicine and Health Sciences
Microscopy
Light Microscopy
Animal Models
Yersinia
Bacterial Pathogens
Body Fluids
Blood
Infectious Diseases
Experimental Organism Systems
Medical Microbiology
Pathogens
Anatomy
Public aspects of medicine
RA1-1270
Plasmids
Research Article
Yersinia Pestis
Hemorrhage
Mouse Models
Research and Analysis Methods
Microbiology
Lymphatic System
Signs and Symptoms
Model Organisms
Animals
Humans
Microbial Pathogens
Plague
Bacteria
Organisms
Biology and Life Sciences
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Plagues
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics
Cardiovascular Anatomy
Animal Studies
Blood Vessels
Lymph Nodes
Clinical Medicine
Subjects
Details
- Language :
- English
- ISSN :
- 19352735 and 19352727
- Volume :
- 15
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS Neglected Tropical Diseases
- Accession number :
- edsair.pmid.dedup....e3da1af099e5510c1144db658ac8e89a