Genome Editing in Mice Using TALE Nucleases

Authors :: Wefers, Benedikt
Brandl, Christina
Ortiz, Oskar
Wurst, Wolfgang
Kühn, Ralf
Kuehn, R.
Wurst, W.
Wefers, B.
Source :: Methods in molecular biology 1338, 229-243 (2016). doi:10.1007/978-1-4939-2932-0_17, TALENs / Kühn, Ralf (Editor) ; New York, NY : Springer New York, 2016, Chapter 17 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-4939-2931-3=978-1-4939-2932-0 ; doi:10.1007/978-1-4939-2932-0
Publication Year :: 2015
Abstract: Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step without the need for embryonic stem cells. By embryo microinjection of TALEN mRNAs and targeting vectors, knockout and knock-in alleles can be generated fast and efficiently. In this chapter we provide protocols for the application of TALENs in mouse zygotes.