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Neural differentiation modulates the vertebrate brain specific splicing program
- Source :
- PLoS ONE, Vol 10, Iss 5, p e0125998 (2015), PLoS ONE, PLoS ONE, Public Library of Science, 2015, 10 (5), pp.e0125998. ⟨10.1371/journal.pone.0125998⟩
- Publication Year :
- 2015
- Publisher :
- Public Library of Science (PLoS), 2015.
-
Abstract
- International audience; Alternative splicing patterns are known to vary between tissues but these patterns have been found to be predominantly peculiar to one species or another, implying only a limited function in fundamental neural biology. Here we used high-throughput RT-PCR to monitor the expression pattern of all the annotated simple alternative splicing events (ASEs) in the Reference Sequence Database, in different mouse tissues and identified 93 brain-specific events that shift from one isoform to another (switch-like) between brain and other tissues. Consistent with an important function, regulation of a core set of 9 conserved switch-like ASEs is highly conserved, as they have the same pattern of tissue-specific splicing in all vertebrates tested: human, mouse and zebrafish. Several of these ASEs are embedded within genes that encode proteins associated with the neuronal microtubule network, and show a dramatic and concerted shift within a short time window of human neural stem cell differentiation. Similarly these exons are dynamically regulated in zebrafish development. These data demonstrate that although alternative splicing patterns often vary between species , there is nonetheless a core set of vertebrate brain-specific ASEs that are conserved between species and associated with neural differentiation.
- Subjects :
- Neurons
animal structures
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
lcsh:R
lcsh:Medicine
Cell Differentiation
Real-Time Polymerase Chain Reaction
Alternative Splicing
Mice
Vertebrates
Animals
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
lcsh:Q
lcsh:Science
[SDV.BDD]Life Sciences [q-bio]/Development Biology
Zebrafish
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 10
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.pmid.dedup....def20ab0464652f07a6ccfea35e95372