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Analysis of sperm aneuploidy by PRINS
- Source :
- Methods in Molecular Biology, Methods in Molecular Biology, Humana Press/Springer Imprint, 2006, 334, pp.49-59. ⟨10.1385/1-59745-068-5:49⟩, Methods in Molecular Biology (Clifton then Totova), Methods in Molecular Biology (Clifton then Totova), Humana Press (Springer Imprint), 2006, 334, pp.49-59
- Publication Year :
- 2006
- Publisher :
- HAL CCSD, 2006.
-
Abstract
- Based on the direct in situ mixing of the colors of different fluorochromes (fluorescein isothiocyanate, tetramethylrhodamine isothiocyanate, Cascade Blue) incorporated in sequential primed in situ labeling (PRINS) reactions, a new multicolor PRINS procedure is described, allowing the rapid and distinct in situ labeling of three or four human chromosomes. Each PRINS reaction consists of a unique 5-min step for annealing and elongation. In combination with the 0.5 M NaOH pretreatment for simultaneous in situ denaturation and decondensation of sperm nuclei, this technique has been adapted to human sperm nuclei for the direct assessment of aneuploidy.
Details
- Language :
- English
- ISSN :
- 10643745 and 19406029
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology, Methods in Molecular Biology, Humana Press/Springer Imprint, 2006, 334, pp.49-59. ⟨10.1385/1-59745-068-5:49⟩, Methods in Molecular Biology (Clifton then Totova), Methods in Molecular Biology (Clifton then Totova), Humana Press (Springer Imprint), 2006, 334, pp.49-59
- Accession number :
- edsair.pmid.dedup....d375244f00cf31de7b30c1c7b328adee
- Full Text :
- https://doi.org/10.1385/1-59745-068-5:49⟩