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Constitutional downregulation of SEMA5A expression in autism
- Source :
- Neuropsychobiology, Neuropsychobiology, 2006, 54 (1), pp.64-9. ⟨10.1159/000096040⟩, Neuropsychobiology, Karger, 2006, 54 (1), pp.64-9. ⟨10.1159/000096040⟩
- Publication Year :
- 2006
- Publisher :
- HAL CCSD, 2006.
-
Abstract
- International audience; There is strong evidence for the importance of genetic factors in idiopathic autism. The results from independent twin and family studies suggest that the disorder is caused by the action of several genes, possibly acting epistatically. We have used cDNA microarray technology for the identification of constitutional changes in the gene expression profile associated with idiopathic autism. Samples were obtained and analyzed from 6 affected subjects belonging to multiplex autism families and from 6 healthy controls. We assessed the expression levels for approximately 7,700 genes by cDNA microarrays using mRNA derived from Epstein-Barr virus-transformed B lymphocytes. The microarray data were analyzed in order to identify up- or downregulation of specific genes. A common pattern with nine downregulated genes was identified among samples derived from individuals with autism when compared to controls. Four of these nine genes encode proteins involved in biological processes associated with brain function or the immune system, and are consequently considered as candidates for genes associated with autism. Quantitative real-time PCR confirms the downregulation of the gene encoding SEMA5A, a protein involved in axonal guidance. Epstein-Barr virus should be considered as a possible source for altered expression, but our consistent results make us suggest SEMA5A as a candidate gene in the etiology of idiopathic autism.
- Subjects :
- Male
MESH: Gene Expression
Adolescent
MESH: Autistic Disorder
Down-Regulation
Nerve Tissue Proteins
[SDV.GEN] Life Sciences [q-bio]/Genetics
Semaphorins
Article
MESH: Down-Regulation
MESH: Child
Humans
Genetic Predisposition to Disease
MESH: Nerve Tissue Proteins
Child
Autistic disorder
MESH: Reverse Transcriptase Pol
Oligonucleotide Array Sequence Analysis
MESH: Adolescent
[SDV.GEN]Life Sciences [q-bio]/Genetics
MESH: Humans
Reverse Transcriptase Polymerase Chain Reaction
MESH: Child, Preschool
MESH: Genetic Predisposition to Disease
Membrane Proteins
MESH: Male
SEMA5A
Child, Preschool
MESH: Oligonucleotide Array Sequence Analysis
Female
Gene expression
Chromosome 7q31
MESH: Membrane Proteins
MESH: Female
cDNA microarrays
Subjects
Details
- Language :
- English
- ISSN :
- 0302282X and 14230224
- Database :
- OpenAIRE
- Journal :
- Neuropsychobiology, Neuropsychobiology, 2006, 54 (1), pp.64-9. ⟨10.1159/000096040⟩, Neuropsychobiology, Karger, 2006, 54 (1), pp.64-9. ⟨10.1159/000096040⟩
- Accession number :
- edsair.pmid.dedup....d0f10ed209ccafa0b39f354065e486c0