Back to Search
Start Over
Hydroxychloroquine partially prevents endothelial dysfunction induced by anti-beta-2-GPI antibodies in an in vivo mouse model of antiphospholipid syndrome
- Source :
- PLoS ONE, Vol 13, Iss 11, p e0206814 (2018), PLoS ONE, PLoS ONE, Public Library of Science, 2018, 13 (11), pp.e0206814. ⟨10.1371/journal.pone.0206814⟩
- Publication Year :
- 2018
- Publisher :
- Public Library of Science (PLoS), 2018.
-
Abstract
- Background Antiphospholipid syndrome is associated with endothelial dysfunction, which leads to thrombosis and early atheroma. Given that hydroxychloroquine has anti-thrombotic properties in lupus, we hypothesized that it could reduce endothelial dysfunction in an animal model of antiphospholipid syndrome. We evaluated the effect of hydroxychloroquine in preventing endothelial dysfunction in a mouse model of antiphospholipid syndrome. Methods Antiphospholipid syndrome was induced by an injection of monoclonal anti-beta-2-GPI antibodies. Vascular reactivity was evaluated in mesenteric resistance arteries isolated from mice 3 weeks (APL3W) after receiving a single injection of anti-beta-2-GPI antibodies and after 3 weeks of daily oral hydroxychloroquine treatment (HCQ3W) compared to control mice (CT3W). We evaluated endothelial dysfunction by measuring acetylcholine-mediated vasodilation. A pharmacological approach was used to evaluate NO synthase uncoupling (tetrahydrobiopterin) and the generation of reactive oxygen species (Tempol). Results Impaired acetylcholine-mediated dilation was evidenced in mice 3 weeks after anti-beta-2-GPI antibodies injection compared to CT3W, by reduced maximal dilation (p
- Subjects :
- Physiology
lcsh:Medicine
Cardiovascular Medicine
Biochemistry
Vascular Medicine
Mice
Immune Physiology
Medicine and Health Sciences
Enzyme-Linked Immunoassays
lcsh:Science
ComputingMilieux_MISCELLANEOUS
Immune System Proteins
Neurochemistry
Animal Models
Neurotransmitters
Arteries
Hematology
Antiphospholipid Syndrome
Antibodies, Anti-Idiotypic
Mesenteric Arteries
Vasodilation
Experimental Organism Systems
beta 2-Glycoprotein I
Cardiovascular Diseases
Anatomy
Neurochemicals
Hydroxychloroquine
Research Article
Immunology
Mouse Models
Research and Analysis Methods
Nitric Oxide
Antibodies
Model Organisms
[SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
Animals
Humans
Endothelium
Immunoassays
Blood Coagulation
Coagulation Disorders
lcsh:R
Biology and Life Sciences
Proteins
Thrombosis
Acetylcholine
Disease Models, Animal
Oxidative Stress
Animal Studies
Immunologic Techniques
Cardiovascular Anatomy
Blood Vessels
lcsh:Q
Neuroscience
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 13
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.pmid.dedup....cab7acc9560c18078924508d7b3da312