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Beyond Uterine Natural Killer Cell Numbers in Unexplained Recurrent Pregnancy Loss: Combined Analysis of CD45, CD56, CD16, CD57, and CD138
- Source :
- Diagnostics, Vol 10, Iss 650, p 650 (2020), Diagnostics, Volume 10, Issue 9
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Changes in the number and cytotoxic potential of uterine Natural Killer (uNK) cells have been associated with reduced fertility. To provide a better characterization of immunophenotypes in the endometrium of women with uRPL (unexplained recurrent pregnancy loss), we examined the applicability of a set of five immune cell markers. The concentration (cells/mm2) of CD45+ leukocytes, CD56+ uNK cells, and CD138+ plasma cells as well as of CD16+ and CD57+ cells, which indicate high cytotoxic uNK cells, were assessed by immunohistochemistry in endometrial biopsies from 61 uRPL patients and 10 controls. Control fertile endometria presented 90&ndash<br />300 CD56+ uNK cells/mm2. uRPL cases were classified in subgroups of low (uRPL-CD56low &lt<br />90 cells/mm2), normal (uRPL-CD56normal 90&ndash<br />300 cells/mm2), and high uNK cell counts (uRPL-CD56high &gt<br />300 cells/mm2). Some cases from the uRPL-CD56low and uRPL-CD56normal subgroups showed elevated proportions of cytotoxic CD16+ and CD57+ cells in relation to CD56+ cells. In the uRPL-CD56high subgroup, the CD57/CD56 ratio was reduced in most samples and the CD16/CD56 ratio was unaltered. Analysis of CD138 excluded the influence of chronic endometritis on these observations. Our results reinforce a link between uRPL and a dysfunctional endometrial environment associated with distinct immune cell profiles.
Details
- Language :
- English
- ISSN :
- 20754418
- Volume :
- 10
- Issue :
- 650
- Database :
- OpenAIRE
- Journal :
- Diagnostics
- Accession number :
- edsair.pmid.dedup....b9b0c42f6ed06e70e170fbf9ef2e64d5