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Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity
- Source :
- Scientific Reports, Scientific Reports, Nature Publishing Group, 2016, 6 (1), pp.37953. ⟨10.1038/srep37953⟩, Scientific Reports, 2016, 6 (1), pp.37953. ⟨10.1038/srep37953⟩
- Publication Year :
- 2016
-
Abstract
- International audience; Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble Siglec-5 variants (sSiglec-5/C4BP and sSiglec-5/Fc) revealed a dose- and calcium-dependent binding to PSGL1. Pre-treatment of PSGL1 with sialidase reduced Siglec-5 binding by 79 ± 4%. In confocal immune-fluorescence assays, we observed that 50% of Peripheral Blood Mononuclear Cells (PBMCs) simultaneously express PSGL1 and Siglec-5. Duolink-proximity ligation analysis demonstrated that PSGL1 and Siglec-5 are in close proximity (
- Subjects :
- MESH: Anti-Inflammatory Agents/pharmacology
E-SELECTIN LIGAND-1
[SDV]Life Sciences [q-bio]
Anti-Inflammatory Agents
MESH: Membrane Glycoproteins/genetics
MESH: Antigens, Differentiation, Myelomonocytic/metabolism
MESH: Lectins/metabolism
MESH: Antigens, CD/pharmacology
MESH: Leukocytes, Mononuclear/drug effects
Lectins
MESH: Animals
MYELOID CELLS
MESH: E-Selectin/metabolism
NEUTROPHILS
IN-VIVO
MESH: Inflammation/drug therapy
Membrane Glycoproteins
MESH: Inflammation/chemically induced
MESH: Inflammation/pathology
MESH: Membrane Glycoproteins/metabolism
MESH: Leukocyte Rolling/physiology
P-Selectin
Female
E-Selectin
MESH: Leukocytes, Mononuclear/metabolism
CD33-RELATED SIGLECS
VON-WILLEBRAND-FACTOR
MESH: Lectins/pharmacology
Antigens, Differentiation, Myelomonocytic
MESH: Antigens, Differentiation, Myelomonocytic/pharmacology
MESH: Tumor Necrosis Factor-alpha/toxicity
GLYCOPROTEIN LIGAND
MESH: Antigens, CD/metabolism
Article
MESH: Mice, Inbred C57BL
Antigens, CD
Animals
Humans
Leukocyte Rolling
Protein Interaction Domains and Motifs
MESH: Antigens, Differentiation, Myelomonocytic/genetics
SIALIC-ACID
Inflammation
MESH: Protein Interaction Domains and Motifs
MESH: Humans
Tumor Necrosis Factor-alpha
MESH: Lectins/genetics
MESH: Solubility
Mice, Inbred C57BL
Disease Models, Animal
Solubility
Leukocytes, Mononuclear
MESH: Antigens, CD/genetics
IMMUNE-SYSTEM
MESH: Disease Models, Animal
MESH: Female
MESH: P-Selectin/metabolism
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.pmid.dedup....b0ddd5764163b44fce0bc2bdd8f26d60
- Full Text :
- https://doi.org/10.1038/srep37953⟩