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The small 6C RNA of Corynebacterium glutamicum is involved in the SOS response

Authors :
Pahlke, Jennifer
Dostálová, Hana
Holátko, Jiří
Degner, Ursula
Bott, Michael
Pátek, Miroslav
Polen, Tino
Source :
RNA Biology, RNA biology 13(9), 848-860 (2016). doi:10.1080/15476286.2016.1205776
Publication Year :
2016
Publisher :
Taylor & Francis, 2016.

Abstract

The 6C RNA family is a class of small RNAs highly conserved in Actinobacteria, including the genera Mycobacterium, Streptomyces and Corynebacterium whose physiological function has not yet been elucidated. We found that strong transcription of the cgb_03605 gene, which encodes 6C RNA in C. glutamicum, was driven by the SigA- and SigB-dependent promoter Pcgb_03605. 6C RNA was detected at high level during exponential growth phase (180 to 240 molcules per cell) which even increased at the entry of the stationary phase. 6C RNA level did not decrease within 240 min after transcription had been stopped with rifampicin, which suggests high 6C RNA stability. The expression of cgb_03605 further increased approximately twofold in the presence of DNA-damaging mitomycin C (MMC) and nearly threefold in the absence of LexA. Deletion of the 6C RNA gene cgb_03605 resulted in a higher sensitivity of C. glutamicum toward MMC and UV radiation. These results indicate that 6C RNA is involved in the DNA damage response. Both 6C RNA level-dependent pausing of cell growth and branched cell morphology in response to MMC suggest that 6C RNA may also be involved in a control of cell division.

Details

Language :
English
ISSN :
15558584 and 15476286
Volume :
13
Issue :
9
Database :
OpenAIRE
Journal :
RNA Biology
Accession number :
edsair.pmid.dedup....9fa21afcaa5ebcc58d7603f465b65ee7
Full Text :
https://doi.org/10.1080/15476286.2016.1205776