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Nuclear Localization of Diacylglycerol Kinase Alpha in K562 Cells Is Involved in Cell Cycle Progression
- Publication Year :
- 2017
-
Abstract
- Phosphatidylinositol (PI) signaling is an essential regulator of cell motility and proliferation. A portion of PI metabolism and signaling takes place in the nuclear compartment of eukaryotic cells, where an array of kinases and phosphatases localize and modulate PI. Among these, Diacylglycerol Kinases (DGKs) are a class of phosphotransferases that phosphorylate diacylglycerol and induce the synthesis of phosphatidic acid. Nuclear DGKalpha modulates cell cycle progression, and its activity or expression can lead to changes in the phosphorylated status of the Retinoblastoma protein, thus, impairing G1/S transition and, subsequently, inducing cell cycle arrest, which is often uncoupled with apoptosis or autophagy induction. Here we report for the first time not only that the DGKalpha isoform is highly expressed in the nuclei of human erythroleukemia cell line K562, but also that its nuclear activity drives K562 cells through the G1/S transition during cell cycle progression. J. Cell. Physiol. 232: 2550-2557, 2017. © 2016 Wiley Periodicals, Inc.
- Subjects :
- Diacylglycerol Kinase
Time Factors
Cell Cycle
K562 Cells
Nucleus
Phosphatidylinositol
Physiology
Clinical Biochemistry
Erythroblastic
Cell Nucleus
Dose-Response Relationship, Drug
Humans
Isoenzymes
Leukemia, Erythroblastic, Acute
Phosphorylation
Protein Kinase Inhibitors
RNA Interference
Retinoblastoma Protein
Signal Transduction
Transfection
Cell Proliferation
G1 Phase Cell Cycle Checkpoints
Cell Biology
Acute
Dose-Response Relationship
Leukemia
Medicine (all)
Nucleus, Dyacylglycerol, Cell Cycle, Erythroleukemia, K562
Drug
Subjects
Details
- Language :
- English
- ISSN :
- 25502557
- Database :
- OpenAIRE
- Accession number :
- edsair.pmid.dedup....959456da29f2620b1d7459c7b6974dae