Back to Search
Start Over
Deletion of phosphatidylserine flippase β-subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration
- Source :
- Zoological Research, Zoological Research, Vol 42, Iss 5, Pp 650-659 (2021)
- Publication Year :
- 2021
- Publisher :
- Science Press, 2021.
-
Abstract
- Phosphatidylserine (PS) is distributed asymmetrically in the plasma membrane of eukaryotic cells. Phosphatidylserine flippase (P4-ATPase) transports PS from the outer leaflet of the lipid bilayer to the inner leaflet of the membrane to maintain PS asymmetry. The β subunit TMEM30A is indispensable for transport and proper function of P4-ATPase. Previous studies have shown that the ATP11A and TMEM30A complex is the molecular switch for myotube formation. However, the role of Tmem30a in skeletal muscle regeneration remains elusive. In the current study, Tmem30a was highly expressed in the tibialis anterior (TA) muscles of dystrophin-null (mdx) mice and BaCl2-induced muscle injury model mice. We generated a satellite cell (SC)-specific Tmem30a conditional knockout (cKO) mouse model to investigate the role of Tmem30a in skeletal muscle regeneration. The regenerative ability of cKO mice was evaluated by analyzing the number and diameter of regenerated SCs after the TA muscles were injured by BaCl2-injection. Compared to the control mice, the cKO mice showed decreased Pax7+ and MYH3+ SCs, indicating diminished SC proliferation, and decreased expression of muscular regulatory factors (MYOD and MYOG), suggesting impaired myoblast proliferation in skeletal muscle regeneration. Taken together, these results demonstrate the essential role of Tmem30a in skeletal muscle regeneration.
- Subjects :
- Genotype
Satellite Cells, Skeletal Muscle
Tmem30a
Article
Dystrophin
Mice
Skeletal muscle regeneration
Animals
Regeneration
Muscle, Skeletal
Cell Proliferation
MyoD Protein
Mice, Knockout
Myosin Heavy Chains
Estrogen Antagonists
Membrane Proteins
PAX7 Transcription Factor
Tamoxifen
Knockout mouse model
QL1-991
Gene Expression Regulation
Atp11a
Mice, Inbred mdx
Myogenin
Zoology
Satellite cell
Subjects
Details
- Language :
- English
- ISSN :
- 20958137
- Volume :
- 42
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Zoological Research
- Accession number :
- edsair.pmid.dedup....8e75865f67b625e7c354ee56336a5eef