Back to Search
Start Over
[Gene amplification and chromosomal rearrangements during acquisition of cellular resistance to the antimetabolite coformycin]
- Source :
- Bulletin du Cancer, Bulletin du Cancer, John Libbey Eurotext, 1994, 81 (5), pp.372-80, Bulletin du Cancer, 1994, 81 (5), pp.372-80
- Publication Year :
- 1994
-
Abstract
- International audience; We studied the early stages of gene amplification in a Chinese hamster cell line and we show that two distinct mechanisms can operate at a single locus. Both of them rely on an unequal segregation of gene copies at mitosis. We conclude that cycles of chromatid breakage, followed by fusion of sister chromatids devoid of a telomere that lead to further breaks in mitosis, have a key role in the coupling of gene amplification and genome remodeling. Rearrangements are first limited to a single chromosome but can then potentially spread to any additional chromosome. Occasionally, a sequence containing the selected gene can be looped out, generating a "double minute" and thus initiating an independent process of extrachromosomal amplification.
- Subjects :
- MESH: Gene Rearrangement
[SDV]Life Sciences [q-bio]
Drug Resistance
MESH: Cricetinae
MESH: Gene Amplification
MESH: Drug Resistance, Multiple
MESH: Interphase
Cricetinae
MESH: Chromosome Aberrations
Animals
MESH: Animals
MESH: In Situ Hybridization, Fluorescence
Interphase
Cells, Cultured
In Situ Hybridization, Fluorescence
Chromosome Aberrations
Gene Rearrangement
Coformycin
MESH: Clone Cells
Gene Amplification
Fibroblasts
Drug Resistance, Multiple
Clone Cells
[SDV] Life Sciences [q-bio]
MESH: Karyotyping
MESH: Fibroblasts
Karyotyping
MESH: Drug Resistance
MESH: Coformycin
MESH: Sister Chromatid Exchange
Sister Chromatid Exchange
MESH: Cells, Cultured
Subjects
Details
- Language :
- French
- ISSN :
- 00074551 and 17696917
- Volume :
- 81
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Bulletin du cancer
- Accession number :
- edsair.pmid.dedup....88bea7a8b4fb9874ce0d24861165f6e9