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Anti-CD3 and anti-CD2-induced T-cell activation in primary Sjögren's syndrome
- Publication Year :
- 1989
-
Abstract
- Because T-cell dysfunctions have been reported in patients with primary Sjögren's syndrome (SS), peripheral blood mononuclear cell (PBMC) proliferation obtained with anti-CD3 and anti-CD2 monoclonal antibodies was evaluated in these patients. Anti-CD3-induced mitogenesis, which varied widely among the patients, was lower in subjects with evidence of anti-SSA and anti-SSB antibodies than in controls. Moreover, the anti-CD2-induced response was depressed in about half the patients and the nonresponders were mainly those with anti-SSA and anti-SSB antibodies. Phorbol myristate acetate, a protein kinase C activator, used alone or added to anti-CD3, induced greater proliferation in patients than in control PBMC. In contrast, exogenous recombinant interleukin 2 (rIL-2) did not significantly enhance the anti-CD2-induced response of patients' PBMC, as it did in normal PBMC. Peripheral blood and parotid T cells from a patient with well-defined primary SS and parotid enlargement also responded poorly to anti-CD2 stimulation. Exogenous rIL-2 restored T-cell proliferation only in the salivary gland cultures of this patient. The present findings suggest that there is a T-cell activation defect in subjects with primary SS, particularly in those with circulating anti-SSA and anti-SSB antibodies. In addition, the difference in the response to IL-2 of peripheral blood and parotid-infiltrating T cells would seem to indicate that T-cell subsets are differently distributed in the blood and inflammation site.
- Subjects :
- Adult
Antigens, Differentiation, T-Lymphocyte
Male
CD3 Complex
T-Lymphocytes
CD2 Antigens
Receptors, Antigen, T-Cell
Antibodies, Monoclonal
Middle Aged
Lymphocyte Activation
Sjogren's Syndrome
Antibodies, Antinuclear
Humans
Interleukin-2
Tetradecanoylphorbol Acetate
Female
Receptors, Immunologic
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.pmid.dedup....87f7a9b6f218b87403641fd13708171c