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Mechanisms of HIV-1 evasion to the antiviral activity of chemokine CXCL12 indicate potential links with pathogenesis
- Source :
- PLoS Pathogens, PLoS Pathogens, Public Library of Science, 2021, 17 (4), pp.e1009526. ⟨10.1371/journal.ppat.1009526⟩, PLoS Pathogens, Vol 17, Iss 4, p e1009526 (2021), PLoS Pathogens, 2021, 17 (4), pp.e1009526. ⟨10.1371/journal.ppat.1009526⟩, Repisalud, Instituto de Salud Carlos III (ISCIII)
- Publication Year :
- 2021
- Publisher :
- HAL CCSD, 2021.
-
Abstract
- HIV-1 infects CD4 T lymphocytes (CD4TL) through binding the chemokine receptors CCR5 or CXCR4. CXCR4-using viruses are considered more pathogenic, linked to accelerated depletion of CD4TL and progression to AIDS. However, counterexamples to this paradigm are common, suggesting heterogeneity in the virulence of CXCR4-using viruses. Here, we investigated the role of the CXCR4 chemokine CXCL12 as a driving force behind virus virulence. In vitro, CXCL12 prevents HIV-1 from binding CXCR4 and entering CD4TL, but its role in HIV-1 transmission and propagation remains speculative. Through analysis of thirty envelope glycoproteins (Envs) from patients at different stages of infection, mostly treatment-naïve, we first interrogated whether sensitivity of viruses to inhibition by CXCL12 varies over time in infection. Results show that Envs resistant (RES) to CXCL12 are frequent in patients experiencing low CD4TL levels, most often late in infection, only rarely at the time of primary infection. Sensitivity assays to soluble CD4 or broadly neutralizing antibodies further showed that RES Envs adopt a more closed conformation with distinct antigenicity, compared to CXCL12-sensitive (SENS) Envs. At the level of the host cell, our results suggest that resistance is not due to improved fusion or binding to CD4, but owes to viruses using particular CXCR4 molecules weakly accessible to CXCL12. We finally asked whether the low CD4TL levels in patients are related to increased pathogenicity of RES viruses. Resistance actually provides viruses with an enhanced capacity to enter naive CD4TL when surrounded by CXCL12, which mirrors their situation in lymphoid organs, and to deplete bystander activated effector memory cells. Therefore, RES viruses seem more likely to deregulate CD4TL homeostasis. This work improves our understanding of the pathophysiology and the transmission of HIV-1 and suggests that RES viruses’ receptors could represent new therapeutic targets to help prevent CD4TL depletion in HIV+ patients on cART.<br />Author summary HIV-1 infects immune cells by binding CD4 and a coreceptor, CCR5 or CXCR4. CXCR4-using viruses are thought to accelerate depletion of CD4 T lymphocytes (CD4TL) and AIDS development. This paradigm, while often true, is however not seen in all patients, suggesting heterogeneity in the virulence of viruses. Here, we show that CXCR4-using viruses can be discriminated on the basis of their resistance to the anti-HIV-1 effect of the CXCR4 chemokine CXCL12. Resistant (RES) viruses are found in patients with low CD4TL levels. They are featured by unique properties of their envelope glycoprotein and the way they use CXCR4. Our data also indicate that RES viruses could contribute to CD4TL depletion. They more effectively kill bystander activated effector memory CD4TL, a subset of CD4TL enriched in HIV+ patients, and enter CD4TL subsets surrounded by CXCL12. Resistance could therefore enhance the ability of viruses to target naive cells and their precursors in lymphoid organs where CXCL12 is present, further hindering CD4TL renewal. Beyond presenting a novel view of CXCR4-using HIV-1 pathogenesis, this work also opens new therapeutic perspectives and increases our knowledge of the still debated role of CXCL12 in HIV-1 transmission.
- Subjects :
- RNA viruses
CD4-Positive T-Lymphocytes
MESH: CD4-Positive T-Lymphocytes / virology
[SDV]Life Sciences [q-bio]
HIV Infections
chemokines
Pathology and Laboratory Medicine
MESH: Viral Envelope Proteins / metabolism
MESH: Virulence Substances
White Blood Cells
Spectrum Analysis Techniques
cell staining
Immunodeficiency Viruses
Viral Envelope Proteins
Animal Cells
Medicine and Health Sciences
Homeostasis
viral tropism
MESH: HIV Infections / physiopathology
Biology (General)
Virulence
T Cells
Chemotaxis
MESH: Antiviral Agents / metabolism
Flow Cytometry
MESH: Chemokine CXCL12 / metabolism
[SDV] Life Sciences [q-bio]
Cell Motility
Medical Microbiology
Spectrophotometry
MESH: Homeostasis
Viruses
Cytophotometry
Pathogens
Cellular Types
Research Article
Cell Physiology
Receptors, CXCR4
QH301-705.5
Immune Cells
Immunology
cloning
MESH: HIV-1 / physiology
MESH: HIV-1 / pathogenicity
Research and Analysis Methods
Microbiology
Antiviral Agents
MESH: HIV Infections / transmission
MESH: Receptors, CXCR4 / metabolism
Retroviruses
Humans
MESH: HIV Infections / virology
Molecular Biology Techniques
cell binding
Microbial Pathogens
Molecular Biology
Blood Cells
cell fusion
MESH: Humans
Lentivirus
Organisms
Biology and Life Sciences
HIV
Cell Biology
RC581-607
Chemokine CXCL12
[SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie
HIV-1
viral pathogens
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Immunologic diseases. Allergy
Subjects
Details
- Language :
- English
- ISSN :
- 15537366 and 15537374
- Database :
- OpenAIRE
- Journal :
- PLoS Pathogens, PLoS Pathogens, Public Library of Science, 2021, 17 (4), pp.e1009526. ⟨10.1371/journal.ppat.1009526⟩, PLoS Pathogens, Vol 17, Iss 4, p e1009526 (2021), PLoS Pathogens, 2021, 17 (4), pp.e1009526. ⟨10.1371/journal.ppat.1009526⟩, Repisalud, Instituto de Salud Carlos III (ISCIII)
- Accession number :
- edsair.pmid.dedup....81b742e2a985b3fcdc056844ca0386d7