Back to Search
Start Over
Establishment of a human myeloma cell line with growth-promoting activity for bone marrow-derived fibroblastoid colony-forming cells
- Source :
- Europe PubMed Central
-
Abstract
- A human myeloma cell line, PCM6, was newly established from peripheral blood of a patient with advanced IgG myeloma by addition of recombinant interleukin-6 (IL-6) in culture. PCM6 cells had a morphology typical of mature plasma cells. Cytogenetic and surface marker studies confirmed that PCM6 cells were identical to fresh myeloma cells. Coculture of PCM6 cells with normal bone marrow mononuclear cells resulted in increased colony size of bone marrow-derived fibroblastoid colony-forming cells (CFU-F). Conditioned medium of PCM6 (PCM6-CM) cells increased the CFU-F colony size in a dose-dependent manner. The activity was labile to trypsin treatment but was heat stable (60 degrees C, 30 minutes). Molecular weight of the activity was approximately 165 kd by Sephacryl S-300 gel filtration. Platelet-derived growth factor (PDGF), epidermal growth factor (EGF), transforming growth factor-beta (TGF-beta), and IL-1 beta were not detectable in the conditioned medium. These findings suggest that in some myeloma cases, bone marrow stroma may be affected by CFU-F growth-promoting activity.
- Subjects :
- Platelet-Derived Growth Factor
Herpesvirus 4, Human
Base Sequence
Dose-Response Relationship, Drug
Epidermal Growth Factor
Interleukin-6
Molecular Sequence Data
Genome, Viral
Fibroblasts
Middle Aged
Hematopoietic Stem Cells
Recombinant Proteins
Bone Marrow
Culture Media, Conditioned
Immunoglobulin G
Antigens, Surface
Chromatography, Gel
Tumor Cells, Cultured
Humans
Female
Trypsin
Multiple Myeloma
Cells, Cultured
Interleukin-1
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Europe PubMed Central
- Accession number :
- edsair.pmid.dedup....7d2e0ddb5fd633be5904f9f503717243