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Comparison of Tumor- and Bone Marrow-Derived Mesenchymal Stromal/Stem Cells from Patients with High-Grade Osteosarcoma
- Source :
- International Journal of Molecular Sciences, Vol 19, Iss 3, p 707 (2018), International Journal of Molecular Sciences, International Journal of Molecular Sciences, MDPI, 2018, 19 (3), pp.707. ⟨10.3390/ijms19030707⟩, International Journal of Molecular Sciences, 2018, 19 (3), pp.707. ⟨10.3390/ijms19030707⟩
- Publication Year :
- 2018
- Publisher :
- MDPI AG, 2018.
-
Abstract
- International audience; Osteosarcoma (OS) is suspected to originate from dysfunctional mesenchymal stromal/stem cells (MSC). We sought to identify OS-derived cells (OSDC) with potential cancer stem cell (CSC) properties by comparing OSDC to MSC derived from bone marrow of patients. This study included in vitro characterization with sphere forming assays, differentiation assays, cytogenetic analysis, and in vivo investigations of their tumorigenicity and tumor supportive capacities. Primary cell lines were isolated from nine high-grade OS samples. All primary cell lines demonstrated stromal cell characteristics. Compared to MSC, OSDC presented a higher ability to form sphere clones, indicating a potential CSC phenotype, and were more efficient at differentiation towards osteoblasts. None of the OSDC displayed the complex chromosome rearrangements typical of high grade OS and none of them induced tumors in immunodeficient mice. However, two OSDC demonstrated focused genomic abnormalities. Three out of seven, and six out of seven OSDC showed a supportive role on local tumor development, and on metastatic progression to the lungs, respectively, when co-injected with OS cells in nude mice. The observation of OS-associated stromal cells with rare genetic abnormalities and with the capacity to sustain tumor progression may have implications for future tumor treatments.
- Subjects :
- Adult
Male
cancer stem cells
Adolescent
Karyotype
Bone Neoplasms
[SDV.CAN]Life Sciences [q-bio]/Cancer
Article
Immunophenotyping
lcsh:Chemistry
Young Adult
[SDV.MHEP.PED] Life Sciences [q-bio]/Human health and pathology/Pediatrics
[SDV.CAN] Life Sciences [q-bio]/Cancer
Bone Marrow
Cell Line, Tumor
osteosarcoma
Tumor Microenvironment
Humans
Neoplasm Metastasis
spheres
lcsh:QH301-705.5
Cells, Cultured
Neoplasm Staging
[SDV.MHEP.PED]Life Sciences [q-bio]/Human health and pathology/Pediatrics
mesenchymal stem cells
Coculture Techniques
lcsh:Biology (General)
lcsh:QD1-999
Neoplastic Stem Cells
Female
Neoplasm Grading
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 14220067 and 16616596
- Volume :
- 19
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.pmid.dedup....7488a3bbb412a7442fbb7c7d5f6ed5ad