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Studies on the mechanism of action of mitomycin C

Authors :
G, Rodighiero
S, Marciani Magno
F, Dell'Acqua
D, Vedaldi
Publication Year :
1978

Abstract

The in vitro formation and properties of the molecular complex between mitomycin C and native DNA were examined by means of various experimental methods; the data obtained indicate that the complex is extremely weak and that the chromophoric moiety of the antibiotic is not involved in its formation. The alkylating activity of mitomycin C was also studied using 3H-mitomycin C; while monofunctional alkylation increases almost in parallel with the concentration of the antibiotic, the difunctional alkylation, causing inter-strand cross-linkages in DNA, rapidly reaches a maximum and then remains constant even when increasing the concentration of the antibiotic and monofunctional alkylation. On the basis of these results, the currently accepted molecular model of the mitomycin--DNA interaction must be revised; a new model of this interaction is presented, which is in better agreement with the properties of mitomycin C and with the latest findings on the subject.

Details

Language :
English
Database :
OpenAIRE
Accession number :
edsair.pmid.dedup....6f8a494a56016c94b65a298322b0a7e9