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Altered expression of urokinase-type plasminogen activator and plasminogen activator inhibitor in high-risk soft tissue sarcomas
- Source :
- Scopus-Elsevier, Europe PubMed Central, DIGITUM. Depósito Digital Institucional de la Universidad de Murcia, instname
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Abstract
- In recent years, classification of soft-tissue sarcomas (STS) has improved with cytogenetic analyses, but their clinical behavior is still not easily predictable. The aim of this study was to detect alterations in the urokinase-type plasminogen system, involved in tumor growth and invasion, by comparing mRNA levels of its components with those of paired normal tissues, and relating them with patient clinical course. Real-time PCR was performed on human STS cell lines and tissues from highly malignant STS, including leiomyosarcomas and malignant fibrous histiocytomas, to evaluate the expression of urokinase-type plasminogen activator (uPA), uPA receptor (uPAR) and plasminogen activator inhibitor-1 (PAI-1). Immunohistochemistry of gene products was also performed. Median mRNA values of all genes studied were higher in tumors than in paired normal tissues. In agreement with data on STS cell lines, significant upregulation for uPA and PAI-1 genes compared to reference values was seen. Moreover, different levels of expression were related to histotype and metastatic phenotype. There was accordance between uPA mRNA and protein expression, while immunodetection of PAI-1 product was weak and scattered. In recent years, classification of soft-tissue sarcomas (STS) has improved with cytogenetic analyses, but their clinical behavior is still not easily predictable. The aim of this study was to detect alterations in the urokinase-type plasminogen system, involved in tumor growth and invasion, by comparing mRNA levels of its components with those of paired normal tissues, and relating them with patient clinical course. Real-time PCR was performed on human STS cell lines and tissues from highly malignant STS, including leiomyosarcomas and malignant fibrous histiocytomas, to evaluate the expression of urokinase-type plasminogen activator (uPA), uPA receptor (uPAR) and plasminogen activator inhibitor-1 (PAI-1). Immunohistochemistry of gene products was also performed. Median mRNA values of all genes studied were higher in tumors than in paired normal tissues. In agreement with data on STS cell lines, significant upregulation for uPA and PAI-1 genes compared to reference values was seen. Moreover, different levels of expression were related to histotype and metastatic phenotype. There was accordance between uPA mRNA and protein expression, while immunodetection of PAI-1 product was weak and scattered.
- Subjects :
- Adult
Male
Time Factors
Time Factor
Cell Culture Techniques
Gene Expression
Receptors, Cell Surface
Polymerase Chain Reaction
Disease-Free Survival
Follow-Up Studie
Receptors, Urokinase Plasminogen Activator
Risk Factors
Cell Line, Tumor
Plasminogen Activator Inhibitor 1
Humans
RNA, Messenger
Neoplasm Metastasis
Aged
Risk Factor
Sarcoma
Middle Aged
Urokinase-Type Plasminogen Activator
Immunohistochemistry
Neoplasm Metastasi
616 - Patología. Medicina clínica. Oncología
Case-Control Studies
Female
Case-Control Studie
Cell Culture Technique
Follow-Up Studies
Human
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Scopus-Elsevier, Europe PubMed Central, DIGITUM. Depósito Digital Institucional de la Universidad de Murcia, instname
- Accession number :
- edsair.pmid.dedup....619227d632a4a5a332f833fcbe030e1b