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Nonequilibrium self-assembly dynamics of icosahedral viral capsids packaging genome or polyelectrolyte
- Source :
- Nature Communications, Nature Communications, Nature Publishing Group, 2018, 9, pp.3071. ⟨10.1038/s41467-018-05426-8⟩, Nature Communications, 2018, 9, pp.3071. ⟨10.1038/s41467-018-05426-8⟩, Nature Communications, Nature Publishing Group, 2018, 9 (1), ⟨10.1038/s41467-018-05426-8⟩, Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018), 'Nature Communications ', vol: 9, pages: 3071-1-3071-9 (2018)
- Publication Year :
- 2018
- Publisher :
- HAL CCSD, 2018.
-
Abstract
- The survival of viruses partly relies on their ability to self-assemble inside host cells. Although coarse-grained simulations have identified different pathways leading to assembled virions from their components, experimental evidence is severely lacking. Here, we use time-resolved small-angle X-ray scattering to uncover the nonequilibrium self-assembly dynamics of icosahedral viral capsids packaging their full RNA genome. We reveal the formation of amorphous complexes via an en masse pathway and their relaxation into virions via a synchronous pathway. The binding energy of capsid subunits on the genome is moderate (~7kBT0, with kB the Boltzmann constant and T0 = 298 K, the room temperature), while the energy barrier separating the complexes and the virions is high (~ 20kBT0). A synthetic polyelectrolyte can lower this barrier so that filled capsids are formed in conditions where virions cannot build up. We propose a representation of the dynamics on a free energy landscape.<br />The mechanism by which virus capsules assemble around RNA to package their genetic material is not clear. Here, the authors observed the assembly of the cowpea chlorotic mottle virus capsid around viral RNA or poly(styrene sulfonic acid) using time-resolved small-angle X-ray scattering measurements.
- Subjects :
- DYNAMICS
Science
viruses
[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]
Static Electricity
Genome, Viral
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Article
Capsid
Scattering, Radiation
lcsh:Science
SELF-ASSEMBLY
[PHYS]Physics [physics]
Vigna
Virus Assembly
X-Rays
Cryoelectron Microscopy
Virion
Hydrogen-Ion Concentration
Bromovirus
Polyelectrolytes
GENOME
VIRUS
RNA
Thermodynamics
lcsh:Q
Capsid Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Database :
- OpenAIRE
- Journal :
- Nature Communications, Nature Communications, Nature Publishing Group, 2018, 9, pp.3071. ⟨10.1038/s41467-018-05426-8⟩, Nature Communications, 2018, 9, pp.3071. ⟨10.1038/s41467-018-05426-8⟩, Nature Communications, Nature Publishing Group, 2018, 9 (1), ⟨10.1038/s41467-018-05426-8⟩, Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018), 'Nature Communications ', vol: 9, pages: 3071-1-3071-9 (2018)
- Accession number :
- edsair.pmid.dedup....60c2ee4695a387b4458ee8c271b5c896