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Inhibition of early steps in the lentiviral replication cycle by cathelicidin host defense peptides
- Source :
- Retrovirology, Vol 2, Iss 1, p 2 (2005), Retrovirology, 2, 2-1-2, Retrovirology, 2, 1, pp. 2-1-2, Retrovirology
- Publication Year :
- 2005
- Publisher :
- BMC, 2005.
-
Abstract
- Contains fulltext : 48016.pdf ( ) (Open Access) BACKGROUND: The antibacterial activity of host defense peptides (HDP) is largely mediated by permeabilization of bacterial membranes. The lipid membrane of enveloped viruses might also be a target of antimicrobial peptides. Therefore, we screened a panel of naturally occurring HDPs representing different classes for inhibition of early, Env-independent steps in the HIV replication cycle. A lentiviral vector-based screening assay was used to determine the inhibitory effect of HDPs on early steps in the replication cycle and on cell metabolism. RESULTS: Human LL37 and porcine Protegrin-1 specifically reduced lentiviral vector infectivity, whereas the reduction of luciferase activities observed at high concentrations of the other HDPs is primarily due to modulation of cellular activity and/ or cytotoxicity rather than antiviral activity. A retroviral vector was inhibited by LL37 and Protegrin-1 to similar extent, while no specific inhibition of adenoviral vector mediated gene transfer was observed. Specific inhibitory effects of Protegrin-1 were confirmed for wild type HIV-1. CONCLUSION: Although Protegrin-1 apparently inhibits an early step in the HIV-replication cycle, cytotoxic effects might limit its use as an antiviral agent unless the specificity for the virus can be improved.
- Subjects :
- Chronic inflammation and autoimmunity [UMCN 4.2]
lcsh:Immunologic diseases. Allergy
Research
Genetic Vectors
Lentivirus
Proteins
Virus Replication
Cell Line
Gram-Positive Cocci
Anti-Infective Agents
Cathelicidins
Transduction, Genetic
Gram-Negative Bacteria
HIV-1
Animals
Humans
Luciferases
Peptides
lcsh:RC581-607
Antimicrobial Cationic Peptides
Subjects
Details
- Language :
- English
- ISSN :
- 17424690
- Volume :
- 2
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Retrovirology
- Accession number :
- edsair.pmid.dedup....5d22d0a059f52ef0e79b3db92bbc6d70