Tunable Ampere phase plate for low dose imaging of biomolecular complexes

Authors :: Tavabi, A. H.
Beleggia, M.
Migunov, V.
Savenko, A.
Oktem, O.
Dunin-Borkowski, R. E.
Pozzi, G.
Source :: Scientific Reports, Vol 8, Iss 1, Pp 1-5 (2018), Scientific Reports, Tavabi, A H, Beleggia, M, Migunov, V, Savenko, A, Öktem, O, Dunin-Borkowski, R E & Pozzi, G 2018, ' Tunable Ampere phase plate for low dose imaging of biomolecular complexes ', Scientific Reports, vol. 8, no. 1, 5592 . https://doi.org/10.1038/s41598-018-23100-3, Scientific reports 8(1), 5592 (2018). doi:10.1038/s41598-018-23100-3
Publication Year :: 2018
Publisher :: Nature Publishing Group, 2018.
Abstract: A novel device that can be used as a tunable support-free phase plate for transmission electron microscopy of weakly scattering specimens is described. The device relies on the generation of a controlled phase shift by the magnetic field of a segment of current-carrying wire that is oriented parallel or antiparallel to the electron beam. The validity of the concept is established using both experimental electron holographic measurements and a theoretical model based on Ampere's law. Computer simulations are used to illustrate the resulting contrast enhancement for studies of biological cells and macromolecules. QC 20180518