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Genetic background matters: a plant–virus gene-for-gene interaction is strongly influenced by genetic contexts
- Source :
- Molecular Plant Pathology, Molecular Plant Pathology, Wiley, 2011, 12 (9), pp.911-920. ⟨10.1111/j.1364-3703.2011.00724.x⟩
- Publication Year :
- 2011
- Publisher :
- HAL CCSD, 2011.
-
Abstract
- International audience; Evolutionary processes responsible for parasite adaptation to their hosts determine our capacity to manage sustainably resistant plant crops. Most plant–parasite interactions studied so far correspond to gene-for-gene models in which the nature of the alleles present at a plant resistance locus and at a pathogen pathogenicity locus determine entirely the outcome of their confrontation. The interaction between the pepper pvr2 resistance locus and Potato virus Y (PVY) genome-linked protein VPg locus obeys this kind of model. Using synthetic chimeras between two parental PVY cDNA clones, we showed that the viral genetic background surrounding the VPg pathogenicity locus had a strong impact on the resistance breakdown capacity of the virus. Indeed, recombination of the cylindrical inclusion (CI) coding region between two PVY cDNA clones multiplied by six the virus capacity to break down the pvr23-mediated resistance. High-throughput sequencing allowed the exploration of the diversity of PVY populations in response to the selection pressure of the pvr23 resistance. The CI chimera, which possessed an increased resistance breakdown capacity, did not show an increased mutation accumulation rate. Instead, selection of the most frequent resistance-breaking mutation seemed to be more efficient for the CI chimera than for the parental virus clone. These results echoed previous observations, which showed that the plant genetic background in which the pvr23 resistance gene was introduced modified strongly the efficiency of selection of resistance-breaking mutations by PVY. In a broader context, the PVY CI coding region is one of the first identified genetic factors to determine the evolvability of a plant virus.
- Subjects :
- DNA, Complementary
genetic control
séquence nucleique
[SDV]Life Sciences [q-bio]
Potyvirus
plant sciences
virus
piment
phytopathogenic virus
potato virus y
lutte génétique
Disease Resistance
Plant Diseases
virus phytopathogène
nucleic sequence
sustainable resistance
Original Articles
virus y de la pomme de terre
viral proteins
dna complementary
plante légumière
chilli pepper
capsicum
mutation
résistance durable
plant proteins
Protein Binding
Subjects
Details
- Language :
- English
- ISSN :
- 14646722 and 13643703
- Database :
- OpenAIRE
- Journal :
- Molecular Plant Pathology, Molecular Plant Pathology, Wiley, 2011, 12 (9), pp.911-920. ⟨10.1111/j.1364-3703.2011.00724.x⟩
- Accession number :
- edsair.pmid.dedup....3ecf46eab560b0b8b42b07c466ec25c6