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In young primary cultures of rabbit kidney cortical collecting ducts intercalated cells originate from principal or undifferentiated cells
- Source :
- European Journal of Cell Biology, European Journal of Cell Biology, Elsevier, 1995, 66 (2), pp.192-9, European Journal of Cell Biology, 1995, 66 (2), pp.192-9, ResearcherID
- Publication Year :
- 1995
- Publisher :
- HAL CCSD, 1995.
-
Abstract
- International audience; The evolution of a primary culture of rabbit kidney cortical collecting tubule was followed over a period of 10 to 11 days. The cell types of this segment were characterized by using monoclonal antibodies, specifically directed against principal (Mab 703) and intercalated (Mab 503) cells of the apical membrane. The activity of a H+ pump ATPase was revealed in Mab 503-labeled cells, confirming that these cultured cells present characteristics of intercalated cells. The primary culture was also stained with peanut agglutinin (PNA), a specific ligand of beta intercalated cells. During the first two days, some cells, mainly Mab 503-labeled cells, disappeared, and cell division did not occur. At 2 days, the culture showed 80% and 18% of Mab 703-labeled and Mab 503-labeled cells, respectively. The first mitoses were observed at 2 days. From two to four days, cell division was nestly in Mab 703-labeled cells and only rarely seen in Mab 503-labeled cells, although during this period the proportion of Mab 703-labeled cells decreased to 44% of cells and that of Mab 503-labeled cells increased to 30%. The labeling with PNA was curious. Up to 2 days, PNA stained Mab 503-labeled cells, but from 4 days it stained other cells, probably dedifferentiated ones. In our culture conditions types of cells other than Mab 703-labeled and Mab 503-labeled cells occurred. First, throughout the life of the culture, some cells were not recognized by any monoclonal antibody; their number varied between 10 and 28% of the total cell number.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Male
MESH: Cell Differentiation
MESH: Hydrogen-Ion Concentration
Kidney Cortex
Arachis
[SDV]Life Sciences [q-bio]
Mitosis
MESH: Rabbits
MESH: Antibodies, Monoclonal
Peanut Agglutinin
MESH: Lectins
Lectins
Animals
MESH: Animals
Kidney Tubules, Collecting
MESH: Kidney Tubules, Collecting
Cells, Cultured
Staining and Labeling
MESH: Arachis
Antibodies, Monoclonal
Cell Differentiation
MESH: Immunohistochemistry
Hydrogen-Ion Concentration
MESH: Mitosis
MESH: Plant Lectins
Immunohistochemistry
MESH: Peanut Agglutinin
MESH: Male
MESH: Kidney Cortex
MESH: Staining and Labeling
Rabbits
Plant Lectins
MESH: Cells, Cultured
Subjects
Details
- Language :
- English
- ISSN :
- 01719335
- Database :
- OpenAIRE
- Journal :
- European Journal of Cell Biology, European Journal of Cell Biology, Elsevier, 1995, 66 (2), pp.192-9, European Journal of Cell Biology, 1995, 66 (2), pp.192-9, ResearcherID
- Accession number :
- edsair.pmid.dedup....3d0bc81910b83d9088526ef2d697d430