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Low-complexity microbiota in the duodenum of children with newly diagnosed ulcerative colitis
- Source :
- PLoS ONE, Vol 12, Iss 10, p e0186178 (2017), PLoS ONE
- Publication Year :
- 2017
- Publisher :
- Public Library of Science (PLoS), 2017.
-
Abstract
- Background Inflammatory bowel disease (IBD) is characterized by gut dysbiosis. To date, the large bowel microbiota has been in focus. However, the microbiota of the small intestine may also be of importance, as the small bowel is a site for the induction and control of mucosal immune responses, which can be modulated by constituents of the local microbiota. Methods Duodenal fluids were collected during diagnostic work-up of treatment-naïve children who were suspected of having IBD. The duodenal fluids were analyzed by pyrosequencing (average of 32,000 reads/sample, read length of 500 nucleotides). After diagnosis, the duodenal microbiota of subjects with ulcerative colitis (N = 8) or Crohn’s disease (N = 5), and non-IBD controls (N = 8) were compared. Results Pyrosequencing revealed that the duodenal microbiota of children with ulcerative colitis contained fewer Operational Taxonomic Units (OTUs) per individual than the duodenal microbiota of the controls (P = 0.005). This reduction in richness of the duodenal microbiota was seen for three major phyla: Firmicutes, Actinobacteria, and Bacteroidetes. Several bacterial genera were detected less frequently in the children with ulcerative colitis than in the non-IBD controls, including Collinsella (P = 0.001), Lactobacillus (P = 0.007), and Bacillus (P = 0.007), as well as a non-identified member of the order Sphingobacteriales (P = 0.007). Conclusions In this pilot study, we show that the duodenal microbiota of children with ulcerative colitis exhibits reduced overall richness, despite the fact that the inflammation is primarily localized to the colon. These results should be corroborated in a larger study.
- Subjects :
- Male
Adolescent
Colon
Duodenum
Immunology
lcsh:Medicine
Crohn's Disease
Pilot Projects
Microbial Genomics
Gastroenterology and Hepatology
Microbiology
digestive system
Autoimmune Diseases
Diagnostic Medicine
Genetics
Medicine and Health Sciences
Ulcerative Colitis
Humans
Child
lcsh:Science
Bacteria
Microbiota
Inflammatory Bowel Disease
lcsh:R
Organisms
Biology and Life Sciences
Genomics
Colitis
digestive system diseases
Gastrointestinal Tract
Medical Microbiology
Case-Control Studies
Clinical Immunology
Colitis, Ulcerative
Female
lcsh:Q
Microbiome
Clinical Medicine
Anatomy
Digestive System
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.pmid.dedup....3aac63280cbf25eb85553c53bda2200a