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MAPK Phosphatase AP2C3 Induces Ectopic Proliferation of Epidermal Cells Leading to Stomata Development in Arabidopsis
- Source :
- PLoS One, 5 (12), PLoS ONE, PLoS ONE, Vol 5, Iss 12, p e15357 (2010)
- Publication Year :
- 2011
-
Abstract
- In plant post-embryonic epidermis mitogen-activated protein kinase (MAPK) signaling promotes differentiation of pavement cells and inhibits initiation of stomata. Stomata are cells specialized to modulate gas exchange and water loss. Arabidopsis MAPKs MPK3 and MPK6 are at the core of the signaling cascade; however, it is not well understood how the activity of these pleiotropic MAPKs is constrained spatially so that pavement cell differentiation is promoted only outside the stomata lineage. Here we identified a PP2C-type phosphatase termed AP2C3 (Arabidopsis protein phosphatase 2C) that is expressed distinctively during stomata development as well as interacts and inactivates MPK3, MPK4 and MPK6. AP2C3 co-localizes with MAPKs within the nucleus and this localization depends on its N-terminal extension. We show that other closely related phosphatases AP2C2 and AP2C4 are also MAPK phosphatases acting on MPK6, but have a distinct expression pattern from AP2C3. In accordance with this, only AP2C3 ectopic expression is able to stimulate cell proliferation leading to excess stomata development. This function of AP2C3 relies on the domains required for MAPK docking and intracellular localization. Concomitantly, the constitutive and inducible AP2C3 expression deregulates E2F-RB pathway, promotes the abundance and activity of CDKA, as well as changes of CDKB1;1 forms. We suggest that AP2C3 downregulates the MAPK signaling activity to help maintain the balance between differentiation of stomata and pavement cells.
- Subjects :
- Stress signaling cascade
Arabidopsis
lcsh:Medicine
Plant Science
Signal transduction
Q1
Cell Fate Determination
Plant Epidermis
Molecular cell biology
Gene Expression Regulation, Plant
Pattern Formation
TRANSCRIPTION FACTOR
lcsh:Science
Promoter Regions, Genetic
GENE-EXPRESSION
Plant Growth and Development
SECRETORY PEPTIDE
Stem Cells
Mechanisms of Signal Transduction
ACTIVATED PROTEIN-KINASE
PLANT DEVELOPMENT
Signaling cascades
Cell Differentiation
Faculty of Science\Biological Science
Signaling in Selected Disciplines
Plants
Up-Regulation
Phenotype
CYCLIN-DEPENDENT KINASES
Research Article
Leafs
MAPK signaling cascades
MAP Kinase Signaling System
EMERGING FUNCTIONS
Arabidopsis Thaliana
Plant Cell Biology
Model Organisms
Plant and Algal Models
Plant Signaling
Cell Lineage
Biology
Cell Proliferation
Cell Nucleus
Arabidopsis Proteins
lcsh:R
fungi
Signal Termination
NEGATIVE REGULATOR
HYPOCOTYL GROWTH
Protein Structure, Tertiary
INNATE IMMUNITY
Mitogen-Activated Protein Kinase Phosphatases
lcsh:Q
Organism Development
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS One, 5 (12), PLoS ONE, PLoS ONE, Vol 5, Iss 12, p e15357 (2010)
- Accession number :
- edsair.pmid.dedup....230533b1faf2b3d8e0b8c23333e9d8a8