Back to Search
Start Over
CD8 T cell deficiency impairs control of Epstein–Barr virus and worsens with age in multiple sclerosis
- Source :
- Journal of Neurology, Neurosurgery, and Psychiatry, ResearcherID
- Publication Year :
- 2011
- Publisher :
- BMJ Group, 2011.
-
Abstract
- Objective: To investigate T cell and antibody immunity to Epstein–Barr virus (EBV) in multiple sclerosis (MS). Methods: Immunoglobulin G (IgG) immunity to EBV nuclear antigen 1 (EBNA1) and viral capsid antigen was measured by enzyme linked immunosorbent assays, and T cell immunity was assessed using enzyme linked immunospot assays to measure the frequency of peripheral blood mononuclear cells (PBMC) producing interferon γ in response to autologous EBV infected B cell lymphoblastoid cell lines (LCL) in 34 EBV seropositive healthy subjects and 34 EBV seropositive patients with MS who had not received immunomodulatory therapy in the previous 3 months. Results: Patients with MS had increased levels of anti-EBNA1 IgG but a decreased frequency of LCL specific T cells compared with healthy subjects. Using purified populations of CD4+ T cells and CD8+ T cells, we showed that the LCL specific response resides predominantly in the CD8+ population, with a frequency 5–7-fold higher than in the CD4+ population. The decreased CD8+ T cell response to LCL in MS was not caused by decreased HLA class I expression by LCL, and LCL from MS patients could be killed normally by HLA matched EBV specific cytotoxic CD8+ T cell clones from healthy subjects. Furthermore, the decreased CD8+ T cell immunity to EBV was not due to a primary defect in the function of CD8+ T cells because EBV specific cytotoxic CD8+ T cell lines could be generated normally from the PBMC of patients with MS. Conclusion: This quantitative deficiency in CD8+ T cell immunity to EBV might be responsible for the accumulation of EBV infected B cells in the brains of patients with MS.
- Subjects :
- Adult
Male
Epstein-Barr Virus Infections
Herpesvirus 4, Human
Multiple Sclerosis
T-Lymphocytes
T cell exhaustion, immunology
Lambert Eaton syndrome
channels
T-Lymphocytes, Regulatory
Epstein–Barr virus
immunology
Leukocyte Count
Age
hemic and lymphatic diseases
Humans
Aged
HMSN (Charcot-Marie-tooth)
EMG (single fibre)
T-Lymphocytes, Helper-Inducer
PostScript
Middle Aged
Research Papers
virology
CD8 T cell
motor neuron disease
neuropathy
Female
neuromuscular
neurophysiology
Subjects
Details
- Language :
- English
- ISSN :
- 1468330X and 00223050
- Volume :
- 83
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of Neurology, Neurosurgery, and Psychiatry
- Accession number :
- edsair.pmid.dedup....1ec01d5d427d915aeddf395a77a5736e