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Seminal plasma modulates the immune-cytokine network in the porcine uterine tissue and pre-ovulatory follicles
- Source :
- PLoS ONE, Vol 13, Iss 8, p e0202654 (2018)
- Publication Year :
- 2018
-
Abstract
- Evidence is emerging that the interaction between male seminal fluid and female tissues promotes fertility, pregnancy, and health of offspring. This includes the acceleration of ovulation in a species known as a spontaneous ovulator, the domestic pig. Earlier studies revealed that seminal plasma acts by a local mechanism in the female pig. The aim of the present study was to examine local short-term and mid-term effects of seminal plasma (SP) on mRNA expression of immunoregulatory genes and transcripts associated with follicle- and oocyte maturation. In the porcine animal model, effects on mRNA expression in the female tract and preovulatory follicles were examined. SP suppressed mRNA expression of Prostaglandin-Endoperoxide Synthase 2 (PTGS2) ipsilateral to the infused uterine horn which was associated with a lower presence of immune cells in the uterine epithelium and lower PTGS2 immunoreaction. Depending on the sampling time (2 h vs. 17 h) and hormonal status, SP altered significant correlative relations of mRNA expression between PTGS2 and the transcripts Tumor Necrosis Factor Alpha, Tumor Necrosis Factor Alpha-Induced Protein 6 and Pentraxin 3 in uterus, granulosa and cumulus cells. A modulatory effect of SP on the oocyte gene network comprising eight oocyte transcripts was observed: uterine exposure to SP induced positive correlations (r >0.08, p
- Subjects :
- Male
Ovulation
Granulosa cells
Neutrophils
Swine
lcsh:Medicine
Epithelium
Ovarian Follicle
Pregnancy
Semen
Animals
Gene Regulatory Networks
RNA, Messenger
lcsh:Science
Cumulus Cells
Granulosa Cells
Interleukin-6
Tumor Necrosis Factor-alpha
Messenger RNA
lcsh:R
Uterus
Serum Amyloid P-Component
C-Reactive Protein
Cyclooxygenase 2
Models, Animal
Oocytes
Cytokines
Female
lcsh:Q
Gene expression
Cell Adhesion Molecules
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, Vol 13, Iss 8, p e0202654 (2018)
- Accession number :
- edsair.pmid.dedup....0dfcd181a9a9bd504f18abc57748328b