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Suppressive effects of transforming growth factor-beta1 produced by hepatocellular carcinoma cell lines on interferon-gamma production by peripheral blood mononuclear cells
- Source :
- Acta medica Okayama. 56(6)
- Publication Year :
- 2003
-
Abstract
- Transforming growth factor-beta1 (TGF-beta1) exerts potent immunosuppressive effects. In this study, we investigated the potential role of TGF-beta1 produced by hepatocellular carcinoma (HCC) cell lines in immunosuppression mechanisms. Using the Mv1Lu cell-growth inhibition assay and an enzyme-linked immunosorbent assay (ELISA), we detected optimal levels of TGF-beta1 in the culture supernatants conditioned by the HCC cell lines PLC/PRF/5, Hep3B, and HepG2. To determine the biological activity of TGF-beta1 in the supernatants, we examined the effects of the culture supernatants on the production of interferon (IFN)-gamma induced during the culture of peripheral blood mononuclear cells (PBMCs) stimulated with interleukin (IL)-12. IFN-gamma production of IL-12-stimulated PBMCs in the 1:1 dilution of the acid-activated conditioned medium of PLC/PRF/5, Hep3B, and HepG2 reduced to 14.7 +/- 0.8, 17.3 +/- 9.0, and 35.9 +/- 14.6%, respectively, compared with the value in the culture with control medium (complete culture medium). These results suggest that HCC cells producing TGF-beta1 may reduce the generation or activation of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells, and thus could enhance their ability to escape immune-mediated surveillance.
- Subjects :
- Male
immunosuppression
Carcinoma, Hepatocellular
transforming growth factor-?1 (TGF-?1)
Liver Neoplasms
hepatocellular carcinoma
Middle Aged
Interleukin-12
digestive system diseases
Transforming Growth Factor beta1
Interferon-gamma
Transforming Growth Factor beta
Immune Tolerance
Leukocytes, Mononuclear
Tumor Cells, Cultured
interferon-? (IFN-?)
Humans
Female
Aged
Subjects
Details
- ISSN :
- 0386300X
- Volume :
- 56
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Acta medica Okayama
- Accession number :
- edsair.pmid.dedup....0adb3dfa9bde78e96b750f845c5bdf73