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Inhibition of LPS-stimulated NO production in mouse macrophage-like cells by tropolones
- Source :
- Anticancer research. 24(6)
- Publication Year :
- 2005
-
Abstract
- We investigated the effect of 27 tropolones on nitric oxide (NO) production by mouse macrophage-like Raw 264.7 cells. All of these compounds failed to stimulate the Raw 264. 7 cells to produce detectable amounts of NO, but inhibited NO production by lipopolysaccharide (LPS)-activated Raw 264.7 cells to various extents. Generally, the ability of tropolones to inhibit LPS-stimulated NO production was inversely related to their cytotoxic activity. Western blot and RT-PCR analyses demonstrated that the most active compound, 2,4-dibromo-7-methoxytropone [21], significantly reduced both the intracellular concentration of iNOS protein and the expression of iNOS mRNA. ESR spectroscopy showed that [21] did not produce radicals under alkaline condition, nor scavenged NO, produced by NOC-7. These data suggested that the inhibitory effect of [21] on NO production might be generated via the inhibition of iNOS expression, rather than a radical-mediated mechanism.
- Subjects :
- Lipopolysaccharides
Reverse Transcriptase Polymerase Chain Reaction
Macrophages
Blotting, Western
Electron Spin Resonance Spectroscopy
Imidazoles
Nitric Oxide Synthase Type II
Free Radical Scavengers
Nitric Oxide
Benzoates
Tropolone
Mice
Structure-Activity Relationship
Animals
Drug Interactions
RNA, Messenger
Nitric Oxide Synthase
Spin Trapping
Subjects
Details
- ISSN :
- 02507005
- Volume :
- 24
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Anticancer research
- Accession number :
- edsair.pmid..........f3c268a7a96026a14a374c3fca6a4b29