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Dedifferentiation of atrial cardiomyocytes as a result of chronic atrial fibrillation
- Publication Year :
- 1997
-
Abstract
- Chronic atrial fibrillation was induced in goats by electrical pacing. After 9 to 23 weeks of sustained atrial fibrillation, the morphology of the atrial structures was examined. The majority of the cardiomyocytes exhibited marked changes in their cellular substructures, with the replacement of sarcomeres by glycogen as the main characteristic. Using immuno-histochemical staining procedures, we assessed the expression and organization of contractile and cytoskeletal proteins in these cases and compared them with the expression and organization of these proteins in normal atria. Part of the atrial cardiomyocytes acquired a dedifferentiated phenotype, as deduced from the re-expression of alpha-smooth muscle actin, the disappearance of cardiotin, and the staining patterns of titin, which resembled those of embryonic cardiomyocytes. From these results we conclude that chronic atrial fibrillation induces myocardial dedifferentiation. This model of chronic atrial fibrillation in goats offers the possibility to study the time course of changes in cardiac structure during sustained atrial fibrillation and after cardioversion.
- Subjects :
- Cell Nucleus
Goats
Myocardium
Cardiac Pacing, Artificial
Muscle Proteins
Cell Differentiation
Muscle, Smooth
macromolecular substances
Immunohistochemistry
Cytoskeletal Proteins
Sarcoplasmic Reticulum
Contractile Proteins
Atrial Fibrillation
Chronic Disease
cardiovascular system
Animals
Connectin
Female
cardiovascular diseases
Heart Atria
Cell Adhesion Molecules
Protein Kinases
Biomarkers
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.pmid..........e7c4e14e7bf35c1ee5c6d8369ab33c45