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Disposable immunoplatforms for the simultaneous determination of biomarkers for neurodegenerative disorders using poly(amidoamine) dendrimer/gold nanoparticle nanocomposite

Authors :
Verónica, Serafín
Claudia A, Razzino
Maria, Gamella
María, Pedrero
Eloy, Povedano
Ana, Montero-Calle
Rodrigo, Barderas
Miguel, Calero
Anderson O, Lobo
Paloma, Yáñez-Sedeño
Susana, Campuzano
José M, Pingarrón
Source :
Analytical and bioanalytical chemistry. 413(3)
Publication Year :
2020

Abstract

Early diagnosis in primary care settings can increase access to therapies and their efficiency as well as reduce health care costs. In this context, we report in this paper the development of a disposable immunoplatform for the rapid and simultaneous determination of two protein biomarkers recently reported to be involved in the pathological process of neurodegenerative disorders (NDD), tau protein (tau), and TAR DNA-binding protein 43 (TDP-43). The methodology involves implementation of a sandwich-type immunoassay on the surface of dual screen-printed carbon electrodes (dSPCEs) electrochemically grafted with p-aminobenzoic acid (p-ABA), which allows the covalent immobilization of a gold nanoparticle-poly(amidoamine) (PAMAM) dendrimer nanocomposite (3D-Au-PAMAM). This scaffold was employed for the immobilization of the capture antibodies (CAbs). Detector antibodies labeled with horseradish peroxidase (HRP) and amperometric detection at - 0.20 V (vs. Ag pseudo-reference electrode) using the H

Details

ISSN :
16182650
Volume :
413
Issue :
3
Database :
OpenAIRE
Journal :
Analytical and bioanalytical chemistry
Accession number :
edsair.pmid..........e5e9bee38b49c3dc41185fad1f848cfe