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TRIP13 promotes the proliferation and invasion of lung cancer cells via the Wnt signaling pathway and epithelial-mesenchymal transition
- Source :
- Journal of molecular histology. 52(1)
- Publication Year :
- 2020
-
Abstract
- Thyroid hormone receptor interactor 13 (TRIP13) is an ATPase that has been found to be overexpressed in many tumors. The aim of this study was to investigate the role of TRIP13 and its mechanism of action in lung cancer. The expression of TRIP13 was examined in lung cancer tissues and corresponding normal lung tissues by western blotting. TRIP13 was overexpressed or knocked down by transient transfection or siRNA interference in lung cancer cells, respectively. The expression of key proteins associated with the Wnt signaling pathway and epithelial-mesenchymal transition (EMT) was assessed. The interaction between TRIP13 and low-density lipoprotein receptor-related protein 6 (LRP6) was examined by co-immunoprecipitation and laser confocal immunofluorescence. Moreover, this study determined the proliferative and invasive ability of cells through colony formation, cell proliferation, and Matrigel invasion assays. The expression of TRIP13 was higher in lung cancer tissues than in normal lung tissues (p = 0.002), and this correlated with poor patient prognosis (p 0.001). In addition, overexpression of TRIP13 enhanced the levels of active β-catenin and target proteins of the Wnt signaling pathways (p 0.05). This study found that TRIP13 can co-localize and bind with LRP6. Furthermore, overexpression of TRIP13 caused the upregulation of N-cadherin, Snail, and vimentin, and the downregulation of E-cadherin (p 0.05). The aforementioned results were reversed after knocking down the expression of TRIP13 (p 0.05). TRIP13 is highly expressed in lung cancers, indicating poor prognosis. overexpression of TRIP13 promotes the proliferative and invasive ability of lung cancer cells via the activation of Wnt signaling pathway and EMT.
- Subjects :
- Epithelial-Mesenchymal Transition
Lung Neoplasms
Cell Line, Tumor
Low Density Lipoprotein Receptor-Related Protein-6
ATPases Associated with Diverse Cellular Activities
Humans
Cell Cycle Proteins
Neoplasm Invasiveness
Survival Analysis
Wnt Signaling Pathway
Tumor Stem Cell Assay
Cell Proliferation
Neoplasm Staging
Subjects
Details
- ISSN :
- 15672387
- Volume :
- 52
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of molecular histology
- Accession number :
- edsair.pmid..........dfb03df43c5460c50db932b71f39884c