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Lack of Association of a Spontaneous Mutation of the Chrm2 Gene with Behavioral and Physiologic Phenotypic Differences in Inbred Mice
- Publication Year :
- 2010
- Publisher :
- American Association for Laboratory Animal Science, 2010.
-
Abstract
- The nucleotide substitution C797T in the Chrm2 gene causes substitution of leucine for proline at position 266 (P266L) of the CHRM2 protein. Because Chrm2 codes for the type 2 muscarinic receptor, this mutation could influence physiologic and behavioral phenotypes of mice. Chrm2 mRNA was not differentially expressed in 2 brain regions with high cholinergic innervation in a mouse strain that does (BALB/cByJ) or does not (C57BL/6J) have the mutation. In addition, strains of mice with and without the C797T point mutation in Chrm2 did not differ significantly in muscarinic binding properties. Variation across strains was detected in terms of acoustic startle, prepulse inhibition, and the physiologic effects of the muscarinic agonist oxotremorine. However, interstrain differences in these measures did not correlate with the presence of the mutation. Although we were unable to associate a measurable phenotype with the Chrm2 mutation, assessment of the mutation on other genetic backgrounds or in the context of other traits might reveal differential effects. Therefore, despite our negative findings, evaluation of characteristics that involve muscarinic function should be undertaken with caution when comparing mice with different alleles of the Chrm2 gene.
- Subjects :
- Male
Reflex, Startle
Molecular Sequence Data
Mutation, Missense
Mouse Models
Binding, Competitive
Body Temperature
Mice
Species Specificity
Heart Rate
Tremor
Animals
Amino Acid Sequence
Alleles
DNA Primers
Analysis of Variance
Mice, Inbred BALB C
Receptor, Muscarinic M2
Base Sequence
Behavior, Animal
Reverse Transcriptase Polymerase Chain Reaction
Oxotremorine
Brain
Sequence Analysis, DNA
Mice, Inbred C57BL
Phenotype
Salivation
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.pmid..........dde99e1bd24507b1025e4fa71b561c82