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RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-Negative Bacteria

Authors :
Zhongrui, Ma
Peng George, Wang
Source :
Methods in molecular biology (Clifton, N.J.). 1954
Publication Year :
2019

Abstract

RecET direct cloning enables obtaining a large DNA region from genome. Here we describe its applications in cloning of polysaccharide gene cluster from gram-negative bacteria. Rapid and exact cloning of polysaccharide gene cluster can be achieved by this method.

Subjects

Subjects :
DNA, Bacterial
Electroporation
Genes, Bacterial
Polysaccharides
Multigene Family
Genetic Vectors
Gram-Negative Bacteria
Restriction Mapping
Escherichia coli
O Antigens
Cloning, Molecular
Polymerase Chain Reaction
Plasmids

Details

ISSN :
19406029
Volume :
1954
Database :
OpenAIRE
Journal :
Methods in molecular biology (Clifton, N.J.)
Accession number :
edsair.pmid..........dcb3d9b1152080f29ec7a9381c661eee

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Authors Abstract Subjects Details