Physiological Comparisons of Pith Callus With Crown-Gall and Genetic Tumors of Nicotiana glauca, N. langsdorffii, and N. glauca-langsdorffii Grown in Vitro. I. Tumor Induction and Proliferation 1

Agrobacterium tumefaciens B-6 and T-37 strains, inoculated into Nicotiana glauca, N. langsdorffii, and their interspecific hybrid, which forms genetic (spontaneous) tumors as well, initiate amorphous tumors from the B-6 strain and organoid tumors (aberrant roots, stems, and buds) from the T-37 strain. In the hybrid, the critical point was to induce crown gall tumors at the site of wounding and not spontaneous genetic tumors. To succeed, this inoculation had to be made at a very early (5-6 leaf stage of development). It is observed that genetic organoid tumors readily formed at the nodes following flowering or leaf abscission. Furthermore, it was noted that genetic tumor derivatives are obtainable from hybrid pith callus or hybrid seedlings cultured in vitro.A marked difference in stem elongation was observed in hybrid tobacco plants inoculated with different strains of Agrobacterium tumefaciens or distilled water, and uninoculated controls. Inoculation of hybrid plants with the B-6-G2 avirulent strain of Agrobacterium tumefaciens stimulated stem elongation over controls, the T-37 inoculated stems were slightly stunted, and the B-6 inoculated stems were quite stunted and succumbed at an early age.