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α₂-Antiplasmin is involved in bone loss induced by ovariectomy in mice
- Source :
- Bone. 79
- Publication Year :
- 2015
-
Abstract
- The mechanism of postmenopausal osteoporosis is not fully understood. α2-Antiplasmin (α2-AP) is the primary inhibitor of plasmin in the fibrinolytic system, but is known to have activities beyond fibrinolysis. However, its role in bone metabolism and the pathogenesis of osteoporosis remains unknown. In the current study, we therefore examined the effects of α2-AP deficiency on ovariectomy (OVX)-induced bone loss by using wild-type and α2-AP-deficient mice. Quantitative computed tomography analysis revealed that α2-AP deficiency blunted OVX-induced trabecular bone loss in mice. Moreover, α2-AP deficiency significantly blunted serum levels of bone-specific alkaline phosphatase, cross-linked C-telopeptide of type I collagen, and interleukin (IL)-1β elevated by OVX. α2-AP treatment elevated the levels of IL-1β and tumor necrosis factor (TNF)-α mRNA in RAW 264.7 cells, although it suppressed osteoclast formation induced by receptor activator of nuclear factor-κB ligand. α2-AP treatment activated ERK1/2 and p38 MAP kinase pathways in RAW 264.7 cells, and these MAP kinase inhibitors antagonized the levels of IL-1β mRNA elevated by α2-AP. The data demonstrate that α2-AP is linked to bone loss due to OVX, through a mechanism that depends in part on the production of IL-1β and TNF-α in monocytes.
- Subjects :
- Mice, Knockout
Tumor Necrosis Factor-alpha
Ovariectomy
Blotting, Western
Interleukin-1beta
Real-Time Polymerase Chain Reaction
p38 Mitogen-Activated Protein Kinases
Antifibrinolytic Agents
Bone and Bones
Mice, Inbred C57BL
Disease Models, Animal
Mice
Animals
Humans
Female
Tomography, X-Ray Computed
Osteoporosis, Postmenopausal
Signal Transduction
Subjects
Details
- ISSN :
- 18732763
- Volume :
- 79
- Database :
- OpenAIRE
- Journal :
- Bone
- Accession number :
- edsair.pmid..........d9bdd56086d748a2f6a626095ef09578