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ATP-binding cassette superfamily transporter gene expression in human primary ovarian carcinoma
- Source :
- Clinical cancer research : an official journal of the American Association for Cancer Research. 8(12)
- Publication Year :
- 2002
-
Abstract
- The purpose of this study is to attempt to characterize patients with unfavorable clinical outcome by the relative mRNA levels of ABC transporter expression in their tumor samples and to examine whether relative mRNA levels of each of the ABC transporters can be a useful predictor of progression-free survival in advanced ovarian carcinoma.We examined tumor samples taken from 30 patients with primary serous papillary adenocarcinoma of the ovary for the expression of MDR1 and MRP1, MRP2, and MRP3 mRNA by using real-time reverse transcription-PCR, and we evaluated its correlation with clinical outcome. All 30 patients were divided into three groups according to clinical outcome after debulking surgery and platinum-based chemotherapy: 8 patients were classified into the unfavorable group; 11 were classified into the favorable group; and 11 were classified into intermediate group.The relative mRNA levels of MRP1 and MRP3 were significantly different among the three groups, and the mRNA levels of MRP1 and MRP3 in the unfavorable group were significantly higher than those in the favorable group by multiple comparison. The relative mRNA levels of MRP1 expression were significantly correlated with those of MRP3 expression. In the 30 patients with serous papillary adenocarcinoma, univariate and multivariate analysis demonstrated that the high relative mRNA levels of MRP1 expression were significantly correlated with a short period of progression-free survival.In patients with advanced ovarian serous papillary adenocarcinoma, these results suggest that patients with an unfavorable clinical outcome are characterized by increased levels of coordinated MRP1 and MRP3 mRNA expression in their tumor samples. Furthermore, a higher level of MRP1 mRNA expression can be a candidate for a useful predictor of a shorter period of progression-free survival.
- Subjects :
- Ovarian Neoplasms
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression
Membrane Transport Proteins
Antineoplastic Agents
Middle Aged
Prognosis
Disease-Free Survival
Drug Resistance, Multiple
Multidrug Resistance-Associated Protein 2
Cystadenocarcinoma, Serous
Immunoenzyme Techniques
Survival Rate
Adenocarcinoma, Papillary
Drug Resistance, Neoplasm
Humans
Female
ATP Binding Cassette Transporter, Subfamily B, Member 1
RNA, Messenger
RNA, Neoplasm
Multidrug Resistance-Associated Proteins
Adenocarcinoma, Clear Cell
DNA Primers
Subjects
Details
- ISSN :
- 10780432
- Volume :
- 8
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Clinical cancer research : an official journal of the American Association for Cancer Research
- Accession number :
- edsair.pmid..........d74eec8630cff1212f952c3a27e2a099