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Inhibition of LPS-stimulated NO production in mouse macrophage-like cells by azulenes
- Source :
- Anticancer research. 24(6)
- Publication Year :
- 2005
-
Abstract
- We investigated the effect of twenty-seven azulenes on nitric oxide (NO) production by mouse macrophage-like Raw 264.7 cells. No azulene derivative alone induced NO production by the Raw 264.7 cells, but inhibited lipopolysaccharide (LPS)-stimulated NO production to various extents. The ability of azulenes to inhibit NO generation by activated macrophages was generally increased when their cytotoxic activity declined. Western blot and RT-PCR analyses demonstrated that the most potent compound, 1,3-difluoroazulene [11], slightly inhibited the expression of inducible NO synthase (iNOS), but only at extremely high concentrations. ESR spectroscopy showed that [11] did not produce radical under alkaline condition, nor scavenged O2- (generated by HX-XOD reaction) or NO (generated by NOC-7). These data suggest that the inhibitory effect of [11] may be produced via a mechanism other than iNOS induction and a radical-mediated mechanism.
- Subjects :
- Lipopolysaccharides
Reverse Transcriptase Polymerase Chain Reaction
Macrophages
Blotting, Western
Electron Spin Resonance Spectroscopy
Imidazoles
Nitric Oxide Synthase Type II
Free Radical Scavengers
Macrophage Activation
Nitric Oxide
Benzoates
Azulenes
Cell Line
Mice
Structure-Activity Relationship
Hydrazines
Animals
RNA, Messenger
Cycloheptanes
Nitric Oxide Synthase
Spin Trapping
Subjects
Details
- ISSN :
- 02507005
- Volume :
- 24
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Anticancer research
- Accession number :
- edsair.pmid..........d1fcb1ad956de9b8bdf74c188519cd19