Bifidobacterium Animalis Subsp. lactis IDCC 4301 Exerts Anti-obesity Effects in High-fat Diet Fed Mice Model by Regulating Lipid Metabolism

Chronic hypernutrition promotes lipid accumulation in the body and excessive lipid accumulation leads to obesity. An increase in the number and size of adipocytes, a characteristic of obesity is closely associated with adipose dysfunction. Recent in vitro and in vivo studies have shown that probiotics may prevent this dysfunction by regulating lipid metabolism. However, the mechanisms of action of probiotics in obesity are not fully understood and their usage for treating obesity remains limited.Bifidobacterium animalis subsp. lactis IDCC 4301 was selected for its anti-obesity potential after evaluating inhibitory activity of pancreatic lipase and cholesterol reducing activity. Next, we investigated the roles of B. lactis IDCC 4301 on lipid metabolism in 3T3-L1 preadipocytes and high-fat diet (HFD)-fed mice. B. lactis IDCC 4301 inhibited cell differentiation and lipid accumulation by suppressing the expression of adipogenic enzymes in 3T3-L1 cells. Moreover, the administration of B. lactis IDCC 4301 resulted in decreased body and adipose tissue weight, improvement in serum lipid levels, and downregulated adipogenic mRNA expression in HFD-fed mice. Additionally, metabolomic analysis suggested that 2-ketobutyrate should be a possible target compound against obesity.B. lactis IDCC 4301 might be used as an alternative treatment for obesity. This article is protected by copyright. All rights reserved.