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Determination of corilagin in rat plasma using a liquid chromatography-electrospray ionization tandem mass spectrometric method
- Source :
- Biomedical chromatography : BMC. 29(10)
- Publication Year :
- 2014
-
Abstract
- A sensitive and simple liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method for the determination of corilagin in rat plasma has been developed. Samples were prepared with protein precipitation method and analyzed with a triple quadrupole tandem mass spectrometer. We employed negative electrospray ionization as the ionization source and the analytes were detected in multiple reaction monitoring mode. Separation was achieved on a C8 column eluted with mobile phase consisting of methanol-0.1% formic acid in a gradient mode at the flow rate of 0.3 mL/min. The total run time was 7.0 min.This method was proved to have good linearity in the concentration range of 2.5-1000.0 ng/mL. The lower limit of quantification of corilagin was 2.5 ng/mL. The intra- and inter-day relative standard deviationa across three validation runs for four concentration levels were both9.8%. The relative error was within ±6.0%. This assay offers advantages in terms of expediency and suitability for the analysis of corilagin in rat plasma. The practical utility of this new HPLC-MS/MS method was confirmed in pilot plasma concentration studies in rats following oral administration.
- Subjects :
- Male
Spectrometry, Mass, Electrospray Ionization
Plant Extracts
Administration, Oral
Reproducibility of Results
Sensitivity and Specificity
Hydrolyzable Tannins
Rats, Sprague-Dawley
Phyllanthus
Glucosides
Limit of Detection
Tandem Mass Spectrometry
Calibration
Animals
Chromatography, High Pressure Liquid
Subjects
Details
- ISSN :
- 10990801
- Volume :
- 29
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Biomedical chromatography : BMC
- Accession number :
- edsair.pmid..........c5740586dd2a0a3f6bfd893f3b96d9ac